CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015806
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 STE20-related kinase adapter protein alpha 
Protein Synonyms/Alias
 STRAD alpha; STE20-related adapter protein; Serologically defined breast cancer antigen NY-BR-96 
Gene Name
 STRADA 
Gene Synonyms/Alias
 LYK5; STRAD 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
91TVNLARYKPTGEYVTubiquitination[1]
239DFPKYSVKVLPWLSPubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
 Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation. 
Sequence Annotation
 DOMAIN 69 379 Protein kinase.
 MOD_RES 329 329 Phosphothreonine; by LKB1.
 MOD_RES 419 419 Phosphothreonine; by LKB1.  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Cell cycle; Chromosomal rearrangement; Complete proteome; Cytoplasm; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 431 AA 
Protein Sequence
MSFLVSKPER IRRWVSEKFI VEGLRDLELF GEQPPGDTRR KTNDASSESI ASFSKQEVMS 60
SFLPEGGCYE LLTVIGKGFE DLMTVNLARY KPTGEYVTVR RINLEACSNE MVTFLQGELH 120
VSKLFNHPNI VPYRATFIAD NELWVVTSFM AYGSAKDLIC THFMDGMNEL AIAYILQGVL 180
KALDYIHHMG YVHRSVKASH ILISVDGKVY LSGLRSNLSM ISHGQRQRVV HDFPKYSVKV 240
LPWLSPEVLQ QNLQGYDAKS DIYSVGITAC ELANGHVPFK DMPATQMLLE KLNGTVPCLL 300
DTSTIPAEEL TMSPSRSVAN SGLSDSLTTS TPRPSNGDSP SHPYHRTFSP HFHHFVEQCL 360
QRNPDARPSA STLLNHSFFK QIKRRASEAL PELLRPVTPI TNFEGSQSQD HSGIFGLVTN 420
LEELEVDDWE F 431 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0030295; F:protein kinase activator activity; IDA:UniProtKB.
 GO:0016772; F:transferase activity, transferring phosphorus-containing groups; IEA:InterPro.
 GO:0032147; P:activation of protein kinase activity; IDA:UniProtKB.
 GO:0007050; P:cell cycle arrest; TAS:Reactome.
 GO:0008286; P:insulin receptor signaling pathway; TAS:Reactome.
 GO:0006611; P:protein export from nucleus; IDA:UniProtKB. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS50011; PROTEIN_KINASE_DOM 
PRINTS