CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031216
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleoporin p54 
Protein Synonyms/Alias
 cDNA FLJ52746, highly similar to Nucleoporin p54 
Gene Name
 NUP54 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
92ERDAILAKWNQLQAFubiquitination[1]
246ELSPAQIKQLLQNPPubiquitination[1, 2, 3]
265PIIWEQAKVDNPDSEubiquitination[1]
273VDNPDSEKLIPVPMVubiquitination[1, 2]
283PVPMVGFKELLRRLKubiquitination[2, 4]
290KELLRRLKVQDQMTKubiquitination[1, 2]
297KVQDQMTKQHQTRLDubiquitination[1]
315EDISELQKNQTTSVAubiquitination[1, 2]
323NQTTSVAKIAQYKRKubiquitination[2]
350IKQEIQRKSGYAIQAubiquitination[1, 2, 4]
380LNAPTQFKGRLNELMubiquitination[1, 2, 3, 4, 5, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 459 AA 
Protein Sequence
MAFNFGAPSG TSGTAAATAA PAGGFGGFGT TSTTAGSAFS FSAPTNTGTT ALGGLFSQPT 60
QAPTQSNQLI NTASALSAPT LLGDERDAIL AKWNQLQAFW GTGKGYFNNN IPPVEFTQEN 120
PFCRFKAVGY SCMPSNKDED GLVVLVFNKK ETEIRSQQQQ LVESLHKVLG GNQTLTVNVE 180
GTKTLPDDQT EVVIYVVERS PNGTSRRVPA TTLYAHFEQA NIKTQLQQLG VTLSMTRTEL 240
SPAQIKQLLQ NPPAGVDPII WEQAKVDNPD SEKLIPVPMV GFKELLRRLK VQDQMTKQHQ 300
TRLDIISEDI SELQKNQTTS VAKIAQYKRK LMDLSHRTLQ VLIKQEIQRK SGYAIQADEE 360
QLRVQLDTIQ GELNAPTQFK GRLNELMSQI RMQNHFGAVR SEERYYIDAD LLREIKQHLK 420
QQQEGLSHLI SIIKDDLEDI KLVEHGLNET IHIRGGVFS 459 
Gene Ontology
 GO:0005643; C:nuclear pore; IEA:InterPro. 
Interpro
 IPR024864; Nup54/Nup57/Nup44.
 IPR025712; Nup54_alpha-helical_dom. 
Pfam
 PF13874; Nup54 
SMART
  
PROSITE
  
PRINTS