CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001198
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase/endoribonuclease IRE1 
Protein Synonyms/Alias
 Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1; hIRE1p; Ire1-alpha; IRE1a; Serine/threonine-protein kinase; Endoribonuclease 
Gene Name
 ERN1 
Gene Synonyms/Alias
 IRE1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
95KNNEGLTKLPFTIPEubiquitination[1]
347VKFDPGLKSKNKLNYubiquitination[1]
349FDPGLKSKNKLNYLRubiquitination[1]
374TPLSASTKMLERFPNubiquitination[1]
633IRYFCTEKDRQFQYIubiquitination[1]
706PNAHGKIKAMISDFGubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto- activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. 
Sequence Annotation
 DOMAIN 571 832 Protein kinase.
 DOMAIN 835 963 KEN.
 NP_BIND 577 585 ATP (By similarity).
 ACT_SITE 688 688 Proton acceptor (By similarity).
 BINDING 599 599 ATP.
 MOD_RES 973 973 Phosphothreonine.
 CARBOHYD 176 176 N-linked (GlcNAc...) (Potential).  
Keyword
 3D-structure; ADP-ribosylation; Alternative splicing; Apoptosis; ATP-binding; Complete proteome; Disulfide bond; Endoplasmic reticulum; Glycoprotein; Hydrolase; Kinase; Magnesium; Membrane; Metal-binding; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Signal; Transcription; Transcription regulation; Transferase; Transmembrane; Transmembrane helix; Unfolded protein response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 977 AA 
Protein Sequence
MPARRLLLLL TLLLPGLGIF GSTSTVTLPE TLLFVSTLDG SLHAVSKRTG SIKWTLKEDP 60
VLQVPTHVEE PAFLPDPNDG SLYTLGSKNN EGLTKLPFTI PELVQASPCR SSDGILYMGK 120
KQDIWYVIDL LTGEKQQTLS SAFADSLCPS TSLLYLGRTE YTITMYDTKT RELRWNATYF 180
DYAASLPEDD VDYKMSHFVS NGDGLVVTVD SESGDVLWIQ NYASPVVAFY VWQREGLRKV 240
MHINVAVETL RYLTFMSGEV GRITKWKYPF PKETEAKSKL TPTLYVGKYS TSLYASPSMV 300
HEGVAVVPRG STLPLLEGPQ TDGVTIGDKG ECVITPSTDV KFDPGLKSKN KLNYLRNYWL 360
LIGHHETPLS ASTKMLERFP NNLPKHRENV IPADSEKKSF EEVINLVDQT SENAPTTVSR 420
DVEEKPAHAP ARPEAPVDSM LKDMATIILS TFLLIGWVAF IITYPLSMHQ QQQLQHQQFQ 480
KELEKIQLLQ QQQQQLPFHP PGDTAQDGEL LDTSGPYSES SGTSSPSTSP RASNHSLCSG 540
SSASKAGSSP SLEQDDGDEE TSVVIVGKIS FCPKDVLGHG AEGTIVYRGM FDNRDVAVKR 600
ILPECFSFAD REVQLLRESD EHPNVIRYFC TEKDRQFQYI AIELCAATLQ EYVEQKDFAH 660
LGLEPITLLQ QTTSGLAHLH SLNIVHRDLK PHNILISMPN AHGKIKAMIS DFGLCKKLAV 720
GRHSFSRRSG VPGTEGWIAP EMLSEDCKEN PTYTVDIFSA GCVFYYVISE GSHPFGKSLQ 780
RQANILLGAC SLDCLHPEKH EDVIARELIE KMIAMDPQKR PSAKHVLKHP FFWSLEKQLQ 840
FFQDVSDRIE KESLDGPIVK QLERGGRAVV KMDWRENITV PLQTDLRKFR TYKGGSVRDL 900
LRAMRNKKHH YRELPAEVRE TLGSLPDDFV CYFTSRFPHL LAHTYRAMEL CSHERLFQPY 960
YFHEPPEPQP PVTPDAL 977 
Gene Ontology
 GO:0030176; C:integral to endoplasmic reticulum membrane; IDA:UniProtKB.
 GO:0005637; C:nuclear inner membrane; IEA:Compara.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0004521; F:endoribonuclease activity; IDA:UniProtKB.
 GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0006987; P:activation of signaling protein activity involved in unfolded protein response; IDA:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0007050; P:cell cycle arrest; ISS:UniProtKB.
 GO:0006917; P:induction of apoptosis; ISS:UniProtKB.
 GO:0006397; P:mRNA processing; IEA:InterPro.
 GO:1900103; P:positive regulation of endoplasmic reticulum unfolded protein response; IMP:UniProtKB.
 GO:0046777; P:protein autophosphorylation; IEA:Compara.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR010513; KEN_dom.
 IPR011009; Kinase-like_dom.
 IPR018391; PQQ_beta_propeller_repeat.
 IPR000719; Prot_kinase_cat_dom.
 IPR006567; PUG-dom.
 IPR027295; Quinonprotein_ADH-like_fam.
 IPR011047; Quinonprotein_ADH-like_supfam.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase
 PF06479; Ribonuc_2-5A 
SMART
 SM00564; PQQ
 SM00580; PUG 
PROSITE
 PS51392; KEN
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS