UniProt Accession

 APTX_HUMAN; Q7Z2E3; A8MTN4; D3DRK9; D3DRL0; Q0P662; Q5T781; Q5T782; Q5T784; Q6JV81; Q6JV82; Q6JV85; Q7Z2F3; Q7Z336; Q7Z5R5; Q7Z6V7; Q7Z6V8; Q9NXM5 

Genbank Protein ID

 AY302067; AY302068; AY302070; AY302071; AY302072; AY302074; AY040777; AY208829; AY208830; AY208831; AY208832; AY208833; AY208834; AY208835; AY208836; AY208837; AY208838; AY208839; AY208840; AY208841; AY208842; AK000164; AK055672; BX538161; AL162590; AL353717; AL162590; AL353717; AL353717; AL162590; AL353717; AL162590; AL353717; AL353717; CH471071; CH471071; CH471071; CH471071; CH471071; CH471071; BC001628; BC032650; BC104881; AJ565850; AJ565851; AJ565852; AJ565853; AJ565854; AJ565855; AJ575566 

Genbank Nucleotide ID

 AAQ74130.1; AAQ74131.1; AAQ74133.1; AAQ74134.1; AAQ74135.1; AAQ74137.1; AAK91768.1; AAP86319.1; AAP86320.1; AAP86321.1; AAP86322.1; AAP86323.1; AAP86324.1; AAP86325.1; AAP86326.1; AAP86327.1; AAP86328.1; AAP86329.1; AAP86330.1; AAP86331.1; AAP86332.1; BAA90985.1; BAG51552.1; CAD98041.1; CAI15549.1; CAI15549.1; CAI15551.1; CAI15551.1; CAI15728.1; CAI15728.1; CAI15730.1; CAI15730.1; CAI15734.1; CAI15735.1; EAW58530.1; EAW58529.1; EAW58531.1; EAW58532.1; EAW58534.1; EAW58535.1; AAH01628.1; AAH32650.1; AAI04882.1; CAD92454.1; CAD92455.1; CAD92456.1; CAD92457.1; CAD92458.1; CAD92459.1; CAE01427.1 

Protein Name

 Aprataxin 

Protein Synonyms/Alias

 Forkhead-associated domain histidine triad-like protein; FHA-HIT 

Gene Name

 APTX 

Gene Synonyms/Alias

 AXA1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
328	DGMPELLKLPLRCHE	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non- ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'- monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity. 

Sequence Annotation

 DOMAIN 38 87 FHA-like.
 DOMAIN 182 287 HIT.
 ZN_FING 331 353 C2H2-type.
 REGION 1 110 Interactions with ADPRT and NCL.
 MOTIF 126 131 Nuclear localization signal (Probable).
 MOTIF 272 276 Histidine triad motif.
 ACT_SITE 274 274 Tele-AMP-histidine intermediate
 MOD_RES 132 132 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Disease mutation; DNA damage; DNA repair; DNA-binding; Hydrolase; Metal-binding; Neurodegeneration; Nucleus; Phosphoprotein; Reference proteome; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 356 AA 

Protein Sequence

Gene Ontology

 GO:0000785; C:chromatin; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; IDA:UniProtKB.
 GO:0003684; F:damaged DNA binding; IDA:UniProtKB.
 GO:0033699; F:DNA 5'-adenosine monophosphate hydrolase activity; IDA:UniProtKB.
 GO:0003690; F:double-stranded DNA binding; IDA:UniProtKB.
 GO:0003725; F:double-stranded RNA binding; IDA:UniProtKB.
 GO:0008967; F:phosphoglycolate phosphatase activity; IDA:UniProtKB.
 GO:0046403; F:polynucleotide 3'-phosphatase activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0008219; P:cell death; IEA:UniProtKB-KW.
 GO:0006266; P:DNA ligation; IEA:Compara.
 GO:0006302; P:double-strand break repair; IDA:UniProtKB.
 GO:0031647; P:regulation of protein stability; IMP:UniProtKB.
 GO:0042542; P:response to hydrogen peroxide; IDA:UniProtKB.
 GO:0000012; P:single strand break repair; IDA:UniProtKB. 

Interpro

 IPR000253; FHA_dom.
 IPR019808; Histidine_triad_CS.
 IPR001310; Histidine_triad_HIT.
 IPR011146; HIT-like.
 IPR008984; SMAD_FHA_domain.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like. 

Pfam

  

SMART

 SM00355; ZnF_C2H2 

PROSITE

 PS00892; HIT_1
 PS51084; HIT_2
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 

PRINTS