CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021229
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rac GTPase-activating protein 1 
Protein Synonyms/Alias
 Male germ cell RacGap; MgcRacGAP; Protein CYK4 homolog; CYK4; HsCYK-4 
Gene Name
 RACGAP1 
Gene Synonyms/Alias
 KIAA1478; MGCRACGAP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
71ERSALDVKLKHARNQubiquitination[1, 2, 3, 4]
84NQVDVEIKRRQRAEAubiquitination[3]
173DWDSSLVKTFKLKKRubiquitination[1, 4]
199DGPPGPVKKTRSIGSubiquitination[1, 4]
200GPPGPVKKTRSIGSAubiquitination[3]
248YWTRSRRKTGTLQPWubiquitination[1, 3, 4]
292RLHDFVSKTVIKPESubiquitination[1, 2, 3, 4, 5, 6]
296FVSKTVIKPESCVPCubiquitination[5]
423HAICSLLKDFLRNLKubiquitination[3]
430KDFLRNLKEPLLTFRubiquitination[1, 4]
490VAQSPHTKMDVANLAubiquitination[1, 3, 4]
522VTMLQDIKRQPKVVEubiquitination[3]
587TPEHQLLKTPSSSSLubiquitination[1, 4, 6]
604RVRSTLTKNTPRFGSubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells. 
Sequence Annotation
 DOMAIN 349 539 Rho-GAP.
 ZN_FING 286 335 Phorbol-ester/DAG-type.
 REGION 106 285 Interaction with SLC26A8.
 MOD_RES 149 149 Phosphoserine; by PLK1.
 MOD_RES 154 154 Phosphoserine.
 MOD_RES 157 157 Phosphoserine; by PLK1.
 MOD_RES 161 161 Phosphothreonine.
 MOD_RES 164 164 Phosphoserine; by PLK1.
 MOD_RES 170 170 Phosphoserine; by PLK1.
 MOD_RES 203 203 Phosphoserine.
 MOD_RES 206 206 Phosphoserine.
 MOD_RES 214 214 Phosphoserine.
 MOD_RES 257 257 Phosphoserine.
 MOD_RES 260 260 Phosphothreonine.
 MOD_RES 342 342 Phosphothreonine.
 MOD_RES 387 387 Phosphoserine; by AURKB.
 MOD_RES 410 410 Phosphoserine; by AURKB.
 MOD_RES 567 567 Phosphothreonine.
 MOD_RES 580 580 Phosphothreonine.
 MOD_RES 588 588 Phosphothreonine.
 MOD_RES 600 600 Phosphoserine.
 MOD_RES 601 601 Phosphothreonine.
 MOD_RES 606 606 Phosphothreonine.  
Keyword
 3D-structure; Cell cycle; Cell division; Coiled coil; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Developmental protein; Differentiation; GTPase activation; Ion transport; Metal-binding; Microtubule; Nucleus; Phosphoprotein; Reference proteome; Spermatogenesis; Transport; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 632 AA 
Protein Sequence
MDTMMLNVRN LFEQLVRRVE ILSEGNEVQF IQLAKDFEDF RKKWQRTDHE LGKYKDLLMK 60
AETERSALDV KLKHARNQVD VEIKRRQRAE ADCEKLERQI QLIREMLMCD TSGSIQLSEE 120
QKSALAFLNR GQPSSSNAGN KRLSTIDESG SILSDISFDK TDESLDWDSS LVKTFKLKKR 180
EKRRSTSRQF VDGPPGPVKK TRSIGSAVDQ GNESIVAKTT VTVPNDGGPI EAVSTIETVP 240
YWTRSRRKTG TLQPWNSDST LNSRQLEPRT ETDSVGTPQS NGGMRLHDFV SKTVIKPESC 300
VPCGKRIKFG KLSLKCRDCR VVSHPECRDR CPLPCIPTLI GTPVKIGEGM LADFVSQTSP 360
MIPSIVVHCV NEIEQRGLTE TGLYRISGCD RTVKELKEKF LRVKTVPLLS KVDDIHAICS 420
LLKDFLRNLK EPLLTFRLNR AFMEAAEITD EDNSIAAMYQ AVGELPQANR DTLAFLMIHL 480
QRVAQSPHTK MDVANLAKVF GPTIVAHAVP NPDPVTMLQD IKRQPKVVER LLSLPLEYWS 540
QFMMVEQENI DPLHVIENSN AFSTPQTPDI KVSLLGPVTT PEHQLLKTPS SSSLSQRVRS 600
TLTKNTPRFG SKSKSATNLG RQGNFFASPM LK 632 
Gene Ontology
 GO:0001669; C:acrosomal vesicle; IEA:UniProtKB-SubCell.
 GO:0097149; C:centralspindlin complex; IDA:UniProtKB.
 GO:0032154; C:cleavage furrow; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0030496; C:midbody; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0051233; C:spindle midzone; IDA:UniProtKB.
 GO:0043014; F:alpha-tubulin binding; IDA:UniProtKB.
 GO:0048487; F:beta-tubulin binding; IDA:UniProtKB.
 GO:0043015; F:gamma-tubulin binding; IDA:UniProtKB.
 GO:0005096; F:GTPase activator activity; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008017; F:microtubule binding; IDA:UniProtKB.
 GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0000915; P:cytokinesis, actomyosin contractile ring assembly; IMP:UniProtKB.
 GO:0007108; P:cytokinesis, initiation of separation; IMP:UniProtKB.
 GO:0009790; P:embryo development; ISS:UniProtKB.
 GO:0007018; P:microtubule-based movement; TAS:Reactome.
 GO:0007405; P:neuroblast proliferation; ISS:UniProtKB.
 GO:0032467; P:positive regulation of cytokinesis; IDA:UniProtKB.
 GO:0051988; P:regulation of attachment of spindle microtubules to kinetochore; IMP:UniProtKB.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007283; P:spermatogenesis; IEP:UniProtKB.
 GO:0051256; P:spindle midzone assembly involved in mitosis; IDA:UniProtKB.
 GO:0008272; P:sulfate transport; IDA:UniProtKB. 
Interpro
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom. 
Pfam
 PF00130; C1_1
 PF00620; RhoGAP 
SMART
 SM00109; C1
 SM00324; RhoGAP 
PROSITE
 PS50238; RHOGAP
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS