| Tag | Content |
|---|
| CPLM ID | CPLM-002894 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Cysteine desulfurase |
Protein Synonyms/Alias | NifS protein homolog; ThiI transpersulfidase |
Gene Name | iscS |
Gene Synonyms/Alias | yfhO; yzzO; b2530; JW2514 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 22 | VDPRVAEKMMQFMTM | acetylation | [1] | | 92 | GAANFYQKKGKHIIT | acetylation | [1] | | 101 | GKHIITSKTEHKAVL | acetylation | [1] | | 105 | ITSKTEHKAVLDTCR | acetylation | [1] | | 135 | RNGIIDLKELEAAMR | acetylation | [1] | | 196 | PIDLSQLKVDLMSFS | acetylation | [1] | | 206 | LMSFSGHKIYGPKGI | acetylation | [1] | | 260 | GEAYRIAKEEMATEM | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Catalyzes the removal of elemental sulfur and selenium atoms from cysteine and selenocysteine to produce alanine. Functions as a sulfur delivery protein for NAD, biotin and Fe-S cluster synthesis. Transfers sulfur on 'Cys-456' of ThiI in a transpersulfidation reaction. Transfers sulfur on 'Cys-19' of TusA in a transpersulfidation reaction. Functions also as a selenium delivery protein in the pathway for the biosynthesis of selenophosphate. |
Sequence Annotation | ACT_SITE 328 328 Cysteine persulfide intermediate.
MOD_RES 206 206 N6-(pyridoxal phosphate)lysine. |
Keyword | 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Pyridoxal phosphate; Reference proteome; Transferase; tRNA processing. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 404 AA |
Protein Sequence | MKLPIYLDYS ATTPVDPRVA EKMMQFMTMD GTFGNPASRS HRFGWQAEEA VDIARNQIAD 60
LVGADPREIV FTSGATESDN LAIKGAANFY QKKGKHIITS KTEHKAVLDT CRQLEREGFE 120
VTYLAPQRNG IIDLKELEAA MRDDTILVSI MHVNNEIGVV QDIAAIGEMC RARGIIYHVD 180
ATQSVGKLPI DLSQLKVDLM SFSGHKIYGP KGIGALYVRR KPRVRIEAQM HGGGHERGMR 240
SGTLPVHQIV GMGEAYRIAK EEMATEMERL RGLRNRLWNG IKDIEEVYLN GDLEHGAPNI 300
LNVSFNYVEG ESLIMALKDL AVSSGSACTS ASLEPSYVLR ALGLNDELAH SSIRFSLGRF 360
TTEEEIDYTI ELVRKSIGRL RDLSPLWEMY KQGVDLNSIE WAHH 404 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0031071; F:cysteine desulfurase activity; IDA:EcoCyc. GO:0030170; F:pyridoxal phosphate binding; IDA:EcoCyc. GO:0009000; F:selenocysteine lyase activity; IDA:EcoCyc. GO:0097163; F:sulfur carrier activity; EXP:EcoCyc. GO:0006534; P:cysteine metabolic process; IEA:InterPro. GO:0016226; P:iron-sulfur cluster assembly; IMP:EcoCyc. GO:0018131; P:oxazole or thiazole biosynthetic process; IMP:EcoCyc. GO:0031119; P:tRNA pseudouridine synthesis; IDA:EcoCyc. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |