CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005447
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Lipopolysaccharide core heptose(I) kinase RfaP 
Protein Synonyms/Alias
  
Gene Name
 rfaP 
Gene Synonyms/Alias
 waaP; b3630; JW3605 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
50SGKSYFLKWHKGTTLacetylation[1]
62TTLKEIIKNLLSLRMacetylation[1]
100KGIGFGEKGLNPLTRacetylation[1]
204VPRRWRDKDLIGLYFacetylation[1]
252LLNMASVKAERIKERacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the phosphorylation of heptose(I) of the outer membrane lipopolysaccharide core (By similarity). 
Sequence Annotation
 ACT_SITE 162 162 Potential.  
Keyword
 ATP-binding; Complete proteome; Kinase; Lipopolysaccharide biosynthesis; Magnesium; Nucleotide-binding; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 265 AA 
Protein Sequence
MVELKEPLAT LWRGKDAFAE VKKLNGEVFR ELETRRTLRF ELSGKSYFLK WHKGTTLKEI 60
IKNLLSLRMP VLGADREWHA IHRLSDVGVD TMKGIGFGEK GLNPLTRASF IITEDLTPTI 120
SLEDYCADWA VNPPDIRVKR MLIARVATMV RKMHTAGINH RDCYICHFLL HLPFTGREDE 180
LKISVIDLHR AQIRAKVPRR WRDKDLIGLY FSSMNIGLTQ RDIWRFMKVY FGMPLRKILS 240
LEQNLLNMAS VKAERIKERT QRKGL 265 
Gene Ontology
 GO:0016020; C:membrane; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016301; F:kinase activity; IDA:EcoCyc.
 GO:0016773; F:phosphotransferase activity, alcohol group as acceptor; IEA:InterPro.
 GO:0009244; P:lipopolysaccharide core region biosynthetic process; IGI:EcoCyc. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR010440; LipoPS_kinase.
 IPR017172; Lsacc_core_hep_kinase_RfaP. 
Pfam
 PF06293; Kdo 
SMART
  
PROSITE
  
PRINTS