UniProt Accession

 YIHI_ECOLI; P0A8H6; P32130; Q2M8G3 

Genbank Protein ID

 D16509; L19201; U00096; AP009048 

Genbank Nucleotide ID

 BAA03960.1; AAB03000.1; AAC76863.1; BAE77443.1 

Protein Name

 Der GTPase-activating protein YihI 

Protein Synonyms/Alias

 GAP 

Gene Name

 yihI 

Gene Synonyms/Alias

 b3866; JW3837 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
79	IPLGVTEKVTKQHKP	acetylation	[1]
82	GVTEKVTKQHKPKSE	acetylation	[1]
90	QHKPKSEKPMLSPQA	acetylation	[1]
158	EEEEEDEKQEDMMRL	acetylation	[1]
158	EEEEEDEKQEDMMRL	pupylation	[2]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli.
 Cerda-Maira FA, McAllister F, Bode NJ, Burns KE, Gygi SP, Darwin KH.
 EMBO Rep. 2011 Jul 8;12(8):863-70. [PMID: 21738222] 

Functional Description

 A GTPase-activating protein (GAP) that modifies Der/EngA GTPase function, negatively regulating cell growth, probably via ribosome assembly. Stimulates the GTPase activity of Der; a construct missing the first 45 residues is even more stimulatory. Does not stimulate 2 other GTPases (ObgE and Era). Overexpression inhibits cell growth; precursor 16S rRNA accumulates, the 23S rRNA is 6-7 bases longer than usual, and 50S ribosomal subunits are improperly assembled, leading to 45S subunits lacking proteins L9, L18 and L25. Overexpression of Der in the same cells suppresses the 50S subunit assembly defect, corroborating that YihI and Der interact. 

Sequence Annotation

 REGION 1 45 Not required for interaction with
 REGION 17 45 Arg/Lys rich.
 REGION 148 157 Asp/Glu rich.  

Keyword

 Complete proteome; GTPase activation; Reference proteome; Ribosome biogenesis. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 169 AA 

Protein Sequence

Gene Ontology

 GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
 GO:0090071; P:negative regulation of ribosome biogenesis; IMP:EcoCyc.
 GO:0043547; P:positive regulation of GTPase activity; IDA:EcoCyc.
 GO:0042254; P:ribosome biogenesis; IEA:UniProtKB-KW. 

Interpro

 IPR007336; Der_GTPase_activator. 

Pfam

 PF04220; YihI 

SMART

  

PROSITE

  

PRINTS