UniProt Accession

 GLYC_YEAST; P37291; D6VY60 

Genbank Protein ID

 L22529; X94607; Z73230; BK006945 

Genbank Nucleotide ID

 AAA21023.1; CAA64305.1; CAA97588.1; DAA09376.1 

Protein Name

 Serine hydroxymethyltransferase, cytosolic 

Protein Synonyms/Alias

 SHMT; Glycine hydroxymethyltransferase; Serine methylase 

Gene Name

 SHM2 

Gene Synonyms/Alias

 SHMT2; YLR058C; L2156 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
29	PEVDSIIKDEIERQK	acetylation	[1]
93	LCQQRALKAFHVTPD	acetylation	[1]
179	IDYDTLEKNAILYRP	acetylation	[1]
179	IDYDTLEKNAILYRP	ubiquitination	[2, 3]
187	NAILYRPKVLVAGTS	ubiquitination	[3]
203	YCRLIDYKRMREIAD	acetylation	[1]
316	QAATPEFKEYQTQVL	acetylation	[1]
316	QAATPEFKEYQTQVL	ubiquitination	[3]
324	EYQTQVLKNAKALES	acetylation	[1]
334	KALESEFKNLGYRLV	acetylation	[1]
377	KINIALNKNSIPGDK	acetylation	[1]
377	KINIALNKNSIPGDK	ubiquitination	[3]
384	KNSIPGDKSALVPGG	acetylation	[1]
418	RIVQYINKAVEFAQQ	ubiquitination	[3]
432	QVQQSLPKDACRLKD	ubiquitination	[3]
456	DVLNTWKKEIYDWAG	ubiquitination	[2, 3]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Interconversion of serine and glycine. 

Sequence Annotation

 MOD_RES 20 20 Phosphothreonine.
 MOD_RES 26 26 Phosphoserine.
 MOD_RES 248 248 N6-(pyridoxal phosphate)lysine (By
 MOD_RES 429 429 Phosphoserine.  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; One-carbon metabolism; Phosphoprotein; Pyridoxal phosphate; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 469 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0043332; C:mating projection tip; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0004372; F:glycine hydroxymethyltransferase activity; IMP:SGD.
 GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
 GO:0006544; P:glycine metabolic process; IEA:InterPro.
 GO:0006563; P:L-serine metabolic process; IEA:InterPro.
 GO:0006730; P:one-carbon metabolic process; IMP:SGD.
 GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway. 

Interpro

 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR015422; PyrdxlP-dep_Trfase_major_sub2.
 IPR001085; Ser_HO-MeTrfase.
 IPR019798; Ser_HO-MeTrfase_PLP_BS. 

Pfam

 PF00464; SHMT 

SMART

  

PROSITE

 PS00096; SHMT 

PRINTS