CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029435
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Centrosomal protein of 78 kDa 
Protein Synonyms/Alias
  
Gene Name
 CEP78 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
6**MIDSVKLRRDSAAubiquitination[1]
63APLLSTLKINKDLPLubiquitination[1]
90DTGSDMNKFCRSRVPubiquitination[1]
102RVPAIRYKDVTFQLCubiquitination[1]
198QGADHMAKILKYQTMubiquitination[2]
201DHMAKILKYQTMRRHubiquitination[1, 2]
317LQNGRSAKSEYQWITubiquitination[1, 2, 3]
329WITSPSVKEPSKTAKubiquitination[1, 2, 3]
351LGSGHKGKATIRIGLubiquitination[1]
361IRIGLATKKPVSSGRubiquitination[1, 2]
374GRKHSLGKEYYAPAPubiquitination[1, 3]
408HRGFPLIKTRDICNQubiquitination[1, 2]
578NPPKEEKKALEDEKPubiquitination[1]
584KKALEDEKPEPKQNAubiquitination[1]
602MQNIQFQKITGDARIubiquitination[1, 2, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 705 AA 
Protein Sequence
MIDSVKLRRD SAADFFSHYE YLCALQNSVP LPAVRACLRE GVLDFNADRL RGVDWAPLLS 60
TLKINKDLPL VSIKSFFQPW LGDTGSDMNK FCRSRVPAIR YKDVTFQLCK ALKGCLSISS 120
VLKNLELNGL ILRERDLTIL AKGLNKSASL VHLSLANCPI GDGGLEIICQ GIKSSITLKT 180
VNFTGCNLTW QGADHMAKIL KYQTMRRHEE TWAESLRYRR PDLDCMAGLR RITLNCNTLI 240
GDLGACAFAD SLSEDLWLRA LDLQQCGLTN EGAKALLEAL ETNTTLVVLD IRKNPLIDHS 300
MMKAVIKKVL QNGRSAKSEY QWITSPSVKE PSKTAKQKRR TIILGSGHKG KATIRIGLAT 360
KKPVSSGRKH SLGKEYYAPA PLPPGVSGFL PWRTAERAKR HRGFPLIKTR DICNQLQQPG 420
FPVTVTVESP SSSEVEEVDD SSESVHEVPE KTSIEQEALQ EKLEECLKQL KEERVIRLKV 480
DKRVSELEHE NAQLRNINFS LSEALHAQSL TNMILDDEGV LGSIENSFQK FHAFLDLLKD 540
AGLGQLATMA GIDQSDFQLL GHPQMTSTVS NPPKEEKKAL EDEKPEPKQN ALGQMQNIQF 600
QKITGDARIP LPLDSFPVPV STPEGLGTSS NNLGVPATEQ RQESFEGFIA RMCSPSPDAT 660
SGTGSQRKEE ELSRNSRSSS EKKTKTGEYT KKHSDKQHPG KDLHS 705 
Gene Ontology
  
Interpro
 IPR026212; Cep78. 
Pfam
  
SMART
  
PROSITE
  
PRINTS
 PR02062; CENTROSOME78.