CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017115
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Peptidase M20 domain-containing protein 2 
Protein Synonyms/Alias
 Aminoacylase-1-like protein 2 
Gene Name
 PM20D2 
Gene Synonyms/Alias
 ACY1L2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
24RSELELLKLRSAECIubiquitination[1, 2, 3, 4, 5, 6]
281YFRAPSMKELQVLTKubiquitination[5]
308SGCTVEIKGGAHDYYubiquitination[5]
321YYNVLPNKSLWKAYMubiquitination[1, 2, 5, 6]
425IREDFKLKLQEEQFVubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 436 AA 
Protein Sequence
MRPGGERPVE GGACNGRSEL ELLKLRSAEC IDEAAERLGA LSRAIWSQPE LAYEEHHAHR 60
VLTHFFEREP PAASWAVQPH YQLPTAFRAE WEPPEARAPS ATPRPLHLGF LCEYDALPGI 120
GHACGHNLIA EVGAAAALGV RGALEGLPRP PPPVKVVVLG TPAEEDGGGK IDLIEAGAFT 180
NLDVVFMAHP SQENAAYLPD MAEHDVTVKY YGKASHSASY PWEGLNALDA AVLAYNNLSV 240
FRQQMKPTWR VHGIIKNGGV KPNIIPSYSE LIYYFRAPSM KELQVLTKKA EDCFRAAALA 300
SGCTVEIKGG AHDYYNVLPN KSLWKAYMEN GRKLGIEFIS EDTMLNGPSG STDFGNVSFV 360
VPGIHPYFHI GSNALNHTEQ YTEAAGSQEA QFYTLRTAKA LAMTALDVIF KPELLEGIRE 420
DFKLKLQEEQ FVNAVE 436 
Gene Ontology
 GO:0016787; F:hydrolase activity; IEA:InterPro. 
Interpro
 IPR017144; Pept_M20D_amidohydro_pred.
 IPR002933; Peptidase_M20.
 IPR011650; Peptidase_M20_dimer. 
Pfam
 PF07687; M20_dimer
 PF01546; Peptidase_M20 
SMART
  
PROSITE
  
PRINTS