CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016654
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tubulin-specific chaperone D 
Protein Synonyms/Alias
 Beta-tubulin cofactor D; Tubulin-folding cofactor D 
Gene Name
 Tbcd 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
213AAAVLVSKFITRPDVubiquitination[1]
221FITRPDVKQRKMASFubiquitination[1]
310RLGLTFLKPKVATWRubiquitination[1]
330RSLAANLKLCAPGKSubiquitination[1]
384VVRWSAAKGIGRMAGubiquitination[1]
1013QSLFEYMKGIQKDAQubiquitination[1]
1017EYMKGIQKDAQVLQSubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Tubulin-folding protein; involved in the first step of the tubulin folding pathway. Modulates microtubule dynamics by capturing GTP-bound beta-tubulin (TUBB). Acts as a GTPase- activating protein (GAP) for ARL2. Its ability to interact with beta tubulin is regulated via its interaction with ARL2. Induces microtubule disruption in absence of ARL2. Increases degradation of beta tubulin, when overexpressed in polarized cells. Promotes epithelial cell detachment, a process antagonized by ARL2. Induces tight adherens and tight junctions disassembly at the lateral cell membrane (By similarity). 
Sequence Annotation
 REPEAT 363 401 HEAT 1.
 REPEAT 599 634 HEAT 2.
 REPEAT 752 788 HEAT 3.
 REPEAT 1106 1142 HEAT 4.  
Keyword
 Cell junction; Cell membrane; Chaperone; Complete proteome; Cytoplasm; GTPase activation; Membrane; Reference proteome; Repeat; Tight junction. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1196 AA 
Protein Sequence
MVLSNEPAAS AAEEEVEDDA LVRASALEAF GESAETRALL RSLPAVHRER ASREVAEERF 60
RVIMDKYQEQ PHLLDPHLEW MMNSLLDLVQ DETSLPDLVH LAFKFLYIIT KVRGYKVFLR 120
LFPHEVANVQ PVLDMFTGQN PKDHETWETR YMLLLWLSVT CLIPFDFSRL DGNLSTQTGE 180
TRVPTMDRIL QIAESYLVVS DKARDAAAVL VSKFITRPDV KQRKMASFLD WSLCTLAHSS 240
FQTIEGVITM DGMLQALAQI FKHGKREDCL PYANTVLQCL DGCRLPESSH TSLRKLGVKL 300
VQRLGLTFLK PKVATWRYQR GCRSLAANLK LCAPGKSDQK LLSDSLTSDG DEDYDVPEGV 360
ETVIEQLLVG LKDKDTVVRW SAAKGIGRMA GRLPRELADD VVGSVLDCFS FQETDKAWHG 420
GCLALAELGR RGLLLPSRLS EVVTVILKAL TYDEKRGACS VGANVRDAAC YVCWAFARAY 480
EPQELTPFVT AISSALVIAA VFDRNVNCRR AASAAFQENV GRQGTFPHGI DILTTADYFA 540
VGNISNCFLI ISVFIAGFQE YTKPMIDHLV SMKINHWDGA IRELSAKALH NLTPQVPEYI 600
AMHVFPALLL MTQSPDLHTR HGAILACAEV TYALYKLATQ SNRLVTDYLD EKAVQSLKQI 660
HQQLCDRHLY RGLGGELMRQ AVCILIEKLS LSRMPFKGDA TVEGWQWLIN DTLRSLHLVS 720
SHSRQQIKEV AVSALTALCS EYYVKEPGEA GSSIAKELIP QYLAELQSPE EMARCGFSSA 780
LGALPGFLLR GHLQQVLSGL RRVTCISPND VSFAEARRDG LKAISRICQT VGVNTRGPPD 840
EVICKENISE VYAALLGCMS DYTTDSRGDV GAWVREAAMT SLMDLMLLLA RTEPVLIEAH 900
ICERVMCCVA QQASEKIDRF RAHAARVFLT LLHFDSPPIP HVPHRQELES LFPRSDVATV 960
NWNAPSQAFP LITQLLGLPT YRYHVLLGLA VSVGGLTEST VRHSTQSLFE YMKGIQKDAQ 1020
VLQSFSETLL KVFEDNLLND RVSVSLLKML DQLLANGCFD IFTAEENHPF CVKLLTLCKE 1080
EIKKSKDIQK LRSSIAVLCG MVQFNGDVRK KILLQLFLLL GHPFPVIRKS TASQVYEMVL 1140
TYSDLVDAEV LDEVMSVLSD TAWDAELPVV REQRNRLCDL LGVPRPQLVP KPIPGS 1196 
Gene Ontology
 GO:0005912; C:adherens junction; ISS:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016328; C:lateral plasma membrane; ISS:UniProtKB.
 GO:0005923; C:tight junction; ISS:UniProtKB.
 GO:0048487; F:beta-tubulin binding; ISS:UniProtKB.
 GO:0005096; F:GTPase activator activity; ISS:UniProtKB.
 GO:0034333; P:adherens junction assembly; ISS:UniProtKB.
 GO:0010812; P:negative regulation of cell-substrate adhesion; ISS:UniProtKB.
 GO:0031115; P:negative regulation of microtubule polymerization; ISS:UniProtKB.
 GO:0007023; P:post-chaperonin tubulin folding pathway; ISS:UniProtKB.
 GO:0070830; P:tight junction assembly; ISS:UniProtKB. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR022577; Tubulin_specific_chaperoneD_C. 
Pfam
 PF12612; TFCD_C 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS