CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007936
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytosolic phospholipase A2 
Protein Synonyms/Alias
 cPLA2; Phospholipase A2 group IVA; Phospholipase A2; Phosphatidylcholine 2-acylhydrolase; Lysophospholipase 
Gene Name
 PLA2G4A 
Gene Synonyms/Alias
 CPLA2; PLA2G4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
316NTTLSSLKEKVNTAQubiquitination[1]
371APDLFGSKFFMGTVVubiquitination[1]
541IYEPLDVKSKKIHVVubiquitination[1]
709LNNIDVIKEAMVESIubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response. 
Sequence Annotation
 DOMAIN 5 106 C2.
 DOMAIN 140 740 PLA2c.
 REGION 1 178 Phospholipid binding (Probable).
 ACT_SITE 228 228 Nucleophile.
 ACT_SITE 549 549 Proton acceptor.
 METAL 40 40 Calcium 1.
 METAL 40 40 Calcium 2.
 METAL 41 41 Calcium 1; via carbonyl oxygen.
 METAL 43 43 Calcium 1.
 METAL 43 43 Calcium 2.
 METAL 65 65 Calcium 1.
 METAL 93 93 Calcium 2.
 METAL 94 94 Calcium 2; via carbonyl oxygen.
 METAL 95 95 Calcium 2.
 MOD_RES 268 268 Phosphothreonine.
 MOD_RES 437 437 Phosphoserine.
 MOD_RES 505 505 Phosphoserine; by MAPK.
 MOD_RES 727 727 Phosphoserine.
 MOD_RES 729 729 Phosphoserine.  
Keyword
 3D-structure; Calcium; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Direct protein sequencing; Hydrolase; Lipid degradation; Lipid metabolism; Metal-binding; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 749 AA 
Protein Sequence
MSFIDPYQHI IVEHQYSHKF TVVVLRATKV TKGAFGDMLD TPDPYVELFI STTPDSRKRT 60
RHFNNDINPV WNETFEFILD PNQENVLEIT LMDANYVMDE TLGTATFTVS SMKVGEKKEV 120
PFIFNQVTEM VLEMSLEVCS CPDLRFSMAL CDQEKTFRQQ RKEHIRESMK KLLGPKNSEG 180
LHSARDVPVV AILGSGGGFR AMVGFSGVMK ALYESGILDC ATYVAGLSGS TWYMSTLYSH 240
PDFPEKGPEE INEELMKNVS HNPLLLLTPQ KVKRYVESLW KKKSSGQPVT FTDIFGMLIG 300
ETLIHNRMNT TLSSLKEKVN TAQCPLPLFT CLHVKPDVSE LMFADWVEFS PYEIGMAKYG 360
TFMAPDLFGS KFFMGTVVKK YEENPLHFLM GVWGSAFSIL FNRVLGVSGS QSRGSTMEEE 420
LENITTKHIV SNDSSDSDDE SHEPKGTENE DAGSDYQSDN QASWIHRMIM ALVSDSALFN 480
TREGRAGKVH NFMLGLNLNT SYPLSPLSDF ATQDSFDDDE LDAAVADPDE FERIYEPLDV 540
KSKKIHVVDS GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDSSPPFKEL LLAEKWAKMN 600
KLPFPKIDPY VFDREGLKEC YVFKPKNPDM EKDCPTIIHF VLANINFRKY RAPGVPRETE 660
EEKEIADFDI FDDPESPFST FNFQYPNQAF KRLHDLMHFN TLNNIDVIKE AMVESIEYRR 720
QNPSRCSVSL SNVEARRFFN KEFLSKPKA 749 
Gene Ontology
 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; NAS:UniProtKB.
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0005794; C:Golgi apparatus; IEA:Compara.
 GO:0005743; C:mitochondrial inner membrane; TAS:Reactome.
 GO:0005509; F:calcium ion binding; IDA:UniProtKB.
 GO:0047498; F:calcium-dependent phospholipase A2 activity; EXP:Reactome.
 GO:0005544; F:calcium-dependent phospholipid binding; IDA:UniProtKB.
 GO:0004622; F:lysophospholipase activity; IEA:EC.
 GO:0019369; P:arachidonic acid metabolic process; TAS:Reactome.
 GO:0050482; P:arachidonic acid secretion; IEA:Compara.
 GO:0035965; P:cardiolipin acyl-chain remodeling; TAS:Reactome.
 GO:0071236; P:cellular response to antibiotic; IEA:Compara.
 GO:0046456; P:icosanoid biosynthetic process; IEA:Compara.
 GO:0006654; P:phosphatidic acid biosynthetic process; TAS:Reactome.
 GO:0036151; P:phosphatidylcholine acyl-chain remodeling; TAS:Reactome.
 GO:0036152; P:phosphatidylethanolamine acyl-chain remodeling; TAS:Reactome.
 GO:0036148; P:phosphatidylglycerol acyl-chain remodeling; TAS:Reactome.
 GO:0036149; P:phosphatidylinositol acyl-chain remodeling; TAS:Reactome.
 GO:0036150; P:phosphatidylserine acyl-chain remodeling; TAS:Reactome.
 GO:0009395; P:phospholipid catabolic process; IEA:InterPro.
 GO:0006663; P:platelet activating factor biosynthetic process; NAS:UniProtKB.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0042127; P:regulation of cell proliferation; IEA:Compara. 
Interpro
 IPR016035; Acyl_Trfase/lysoPLipase.
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR002642; LysoPLipase_cat_dom. 
Pfam
 PF00168; C2
 PF01735; PLA2_B 
SMART
 SM00239; C2
 SM00022; PLAc 
PROSITE
 PS50004; C2
 PS51210; PLA2C 
PRINTS