CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011865
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 6-phosphofructo-2-kinase 2 
Protein Synonyms/Alias
 6PF-2-K 2; Phosphofructokinase 2 II 
Gene Name
 PFK27 
Gene Synonyms/Alias
 YOL136C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
55SVFFDNHKSSLLSTEubiquitination[1, 2]
81LPITLNYKRDNADPTubiquitination[2]
150ISCATISKPLLLSNTubiquitination[2]
Reference
 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Synthesis of fructose 2,6-bisphosphate. 
Sequence Annotation
 NP_BIND 103 110 ATP (Potential).
 REGION 85 305 6-phosphofructo-2-kinase.
 ACT_SITE 197 197 Potential.
 ACT_SITE 235 235 Potential.
 BINDING 269 269 Fructose-6-phosphate (By similarity).  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 397 AA 
Protein Sequence
MGGSSDSDSH DGYLTSEYNS SNSLFSLNTG NSYSSASLDR ATLDCQDSVF FDNHKSSLLS 60
TEVPRFISND PLHLPITLNY KRDNADPTYT NGKVNKFMIV LIGLPATGKS TISSHLIQCL 120
KNNPLTNSLR CKVFNAGKIR RQISCATISK PLLLSNTSSE DLFNPKNNDK KETYARITLQ 180
KLFHEINNDE CDVGIFDATN STIERRRFIF EEVCSFNTDE LSSFNLVPII LQVSCFNRSF 240
IKYNIHNKSF NEDYLDKPYE LAIKDFAKRL KHYYSQFTPF SLDEFNQIHR YISQHEEIDT 300
SLFFFNVINA GVVEPHSLNQ SHYPSTCGKQ IRDTIMVIEN FINHYSQMFG FEYIEAVKLF 360
FESFGNSSEE TLTTLDSVVN DKFFDDLQSL IESNGFA 397 
Gene Ontology
 GO:0005737; C:cytoplasm; IC:SGD.
 GO:0003873; F:6-phosphofructo-2-kinase activity; ISS:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0006003; P:fructose 2,6-bisphosphate metabolic process; IMP:SGD.
 GO:0006000; P:fructose metabolic process; IEA:InterPro.
 GO:0006110; P:regulation of glycolysis; IMP:SGD. 
Interpro
 IPR003094; 6Pfruct_kin.
 IPR013079; 6Phosfructo_kin.
 IPR027417; P-loop_NTPase.
 IPR014379; PFK27. 
Pfam
 PF01591; 6PF2K 
SMART
  
PROSITE
  
PRINTS