CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000962
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitotic checkpoint serine/threonine-protein kinase BUB1 beta 
Protein Synonyms/Alias
 MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1 
Gene Name
 BUB1B 
Gene Synonyms/Alias
 BUBR1; MAD3L; SSK1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
59NNTLQQQKRAFEYEIubiquitination[1]
127VEALQGEKRYYSDPRubiquitination[1]
196FQEGIQQKAEPLERLubiquitination[1]
264IRVGGALKAPSQNRGubiquitination[1]
384NHILSTRKPGKEEGDubiquitination[1]
468EEMEKKLKEIQTTQQubiquitination[1]
489QEETMPTKETTKLQIubiquitination[1]
502QIASESQKIPGMTLSubiquitination[1]
554EFLLSEKKNKSPPADubiquitination[1]
643FHEIMSLKDLPSDPEubiquitination[1]
682SQTLSIKKLSPIIEDubiquitination[1]
771MPKLEIEKEIELGNEubiquitination[1]
783GNEDYCIKREYLICEubiquitination[1]
1009KDGELWNKFFVRILNubiquitination[2]
1051HLNKALWKVGKLTSPubiquitination[1]
1054KALWKVGKLTSPGALubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. 
Sequence Annotation
 DOMAIN 62 226 BUB1 N-terminal.
 DOMAIN 766 1050 Protein kinase.
 NP_BIND 772 780 ATP (By similarity).
 REGION 152 185 Necessary for interaction with CASC5.
 MOTIF 111 118 Nuclear localization signal (Potential).
 MOTIF 224 232 D-box.
 ACT_SITE 882 882 Proton acceptor (By similarity).
 BINDING 795 795 ATP (By similarity).
 MOD_RES 250 250 N6-acetyllysine; by PCAF.
 MOD_RES 435 435 Phosphoserine.
 MOD_RES 543 543 Phosphoserine.
 MOD_RES 670 670 Phosphoserine.
 MOD_RES 676 676 Phosphoserine; by PLK1.
 MOD_RES 792 792 Phosphothreonine; by PLK1.
 MOD_RES 1008 1008 Phosphothreonine; by PLK1.
 MOD_RES 1042 1042 Phosphothreonine.
 MOD_RES 1043 1043 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Apoptosis; ATP-binding; Cell cycle; Cell division; Centromere; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Disease mutation; Kinase; Kinetochore; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Tumor suppressor; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1050 AA 
Protein Sequence
MAAVKKEGGA LSEAMSLEGD EWELSKENVQ PLRQGRIMST LQGALAQESA CNNTLQQQKR 60
AFEYEIRFYT GNDPLDVWDR WVFLFHKDNR NINRYISWTE QNYPQGGKES NMSTLLERAV 120
EALQGEKRYY SDPRFLNLWL KLGRLCNEPL DMYSYLHNQG IGVSLAQFYI SWAEEYEARE 180
NFRKADAIFQ EGIQQKAEPL ERLQSQHRQF QARVSRQTLL ALEKEEEEEV FESSVPQRST 240
LAELKSKGKK TARAPIIRVG GALKAPSQNR GLQNPFPQQM QNNSRITVFD ENADEASTAE 300
LSKPTVQPWI APPMPRAKEN ELQAGPWNTG RSLEHRPRGN TASLIAVPAV LPSFTPYVEE 360
TARQPVMTPC KIEPSINHIL STRKPGKEEG DPLQRVQSHQ QASEEKKEKM MYCKEKIYAG 420
VGEFSFEEIR AEVFRKKLKE QREAELLTSA EKRAEMQKQI EEMEKKLKEI QTTQQERTGD 480
QQEETMPTKE TTKLQIASES QKIPGMTLSS SVCQVNCCAR ETSLAENIWQ EQPHSKGPSV 540
PFSIFDEFLL SEKKNKSPPA DPPRVLAQRR PLAVLKTSES ITSNEDVSPD VCDEFTGIEP 600
LSEDAIITGF RNVTICPNPE DTCDFARAAR FVSTPFHEIM SLKDLPSDPE RLLPEEDLDV 660
KTSEDQQTAC GTIYSQTLSI KKLSPIIEDS REATHSSGFS GSSASVASTS SIKCLQIPEK 720
LELTNETSEN PTQSPWCSQY RRQLLKSLPE LSASAELCIE DRPMPKLEIE KEIELGNEDY 780
CIKREYLICE DYKLFWVAPR NSAELTVIKV SSQPVPWDFY INLKLKERLN EDFDHFCSCY 840
QYQDGCIVWH QYINCFTLQD LLQHSEYITH EITVLIIYNL LTIVEMLHKA EIVHGDLSPR 900
CLILRNRIHD PYDCNKNNQA LKIVDFSYSV DLRVQLDVFT LSGFRTVQIL EGQKILANCS 960
SPYQVDLFGI ADLAHLLLFK EHLQVFWDGS FWKLSQNISE LKDGELWNKF FVRILNANDE 1020
ATVSVLGELA AEMNGVFDTT FQSHLNKALW KVGKLTSPGA LLFQ 1064 
Gene Ontology
 GO:0005680; C:anaphase-promoting complex; TAS:ProtInc.
 GO:0000940; C:condensed chromosome outer kinetochore; IDA:UniProtKB.
 GO:0000778; C:condensed nuclear chromosome kinetochore; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL.
 GO:0051233; C:spindle midzone; NAS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004672; F:protein kinase activity; NAS:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; TAS:Reactome.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0007091; P:metaphase/anaphase transition of mitotic cell cycle; IEA:Compara.
 GO:0007067; P:mitosis; NAS:UniProtKB.
 GO:0007094; P:mitotic spindle assembly checkpoint; TAS:Reactome.
 GO:0051436; P:negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
 GO:0048015; P:phosphatidylinositol-mediated signaling; NAS:UniProtKB.
 GO:0071459; P:protein localization to chromosome, centromeric region; IEA:Compara.
 GO:0034501; P:protein localization to kinetochore; IPI:UniProtKB.
 GO:0007051; P:spindle organization; NAS:UniProtKB. 
Interpro
 IPR015661; Bub1/Mad3.
 IPR011009; Kinase-like_dom.
 IPR013212; Mad3_BUB1_I.
 IPR000719; Prot_kinase_cat_dom. 
Pfam
 PF08311; Mad3_BUB1_I
 PF00069; Pkinase 
SMART
 SM00777; Mad3_BUB1_I 
PROSITE
 PS51489; BUB1_N
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS