CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007509
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Coatomer subunit beta 
Protein Synonyms/Alias
 Beta-coat protein; Beta-COP 
Gene Name
 SEC26 
Gene Synonyms/Alias
 YDR238C; YD8419.05C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
40EKGSDEQKIDTMKSIubiquitination[1]
363DVVQLLKKELQTTVNubiquitination[1]
376VNNPDQDKAMQYRQLubiquitination[1]
613GQSSLVEKKIDEDSLubiquitination[1]
614QSSLVEKKIDEDSLEubiquitination[2]
679RALKDSCKNIEPIDTubiquitination[1]
868NAFEWENKISVKSQLubiquitination[1]
887AYLRELVKGTNMGILubiquitination[1]
946GYVRIRSKGQGLALSubiquitination[1]
967LIAKKTNKLALTHV*acetylation[3]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis.
 Ziv I, Matiuhin Y, Kirkpatrick DS, Erpapazoglou Z, Leon S, Pantazopoulou M, Kim W, Gygi SP, Haguenauer-Tsapis R, Reis N, Glickman MH, Kleifeld O.
 Mol Cell Proteomics. 2011 May;10(5):M111.009753. [PMID: 21427232]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Required for mitochondrial morphology. 
Sequence Annotation
 REPEAT 98 133 HEAT 1.
 REPEAT 134 170 HEAT 2.
 REPEAT 279 317 HEAT 3.
 REPEAT 318 354 HEAT 4.
 MOD_RES 181 181 Phosphoserine.
 MOD_RES 540 540 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; Cytoplasmic vesicle; Direct protein sequencing; ER-Golgi transport; Golgi apparatus; Membrane; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 973 AA 
Protein Sequence
MTSLSSQPAY TLVFDPSPSM ETYSSTDFQK ALEKGSDEQK IDTMKSILVT MLEGNPMPEL 60
LMHIIRFVMP SKNKELKKLL YFYWEIVPKL AEDGKLRHEM ILVCNAIQHD LQHPNEYIRG 120
NTLRFLTKLR EAELLEQMVP SVLACLEYRH AYVRKYAILA VFSIFKVSEH LLPDAKEIIN 180
SFIVAETDPI CKRNAFIGLA ELDRENALHY LENNIADIEN LDPLLQAVFV QFIRQDANRT 240
PALKAQYIEL LMELLSTTTS DEVIFETALA LTVLSANPNV LVPAVNKLID LAVKVSDNNI 300
KLIVLDRIQD INANNVGALE ELTLDILRVL NAEDLDVRSK ALDISMDLAT SRNAEDVVQL 360
LKKELQTTVN NPDQDKAMQY RQLLIKTIRT VAVNFVEMAA SVVSLLLDFI GDLNSVAASG 420
IIAFIKEVIE KYPQLRANIL ENMVQTLDKV RSAKAYRGAL WIMGEYAEGE SEIQHCWKHI 480
RNSVGEVPIL QSEIKKLTQN QEHTEENEVD ATAKPTGPVI LPDGTYATES AFDVKTSQKS 540
VTDEERDSRP PIRRFVLSGD FYTAAILANT IIKLVLKFEN VSKNKTVINA LKAEALLILV 600
SIVRVGQSSL VEKKIDEDSL ERVMTSISIL LDEVNPEEKK EEVKLLEVAF LDTTKSSFKR 660
QIEIAKKNKH KRALKDSCKN IEPIDTPISF RQFAGVDSTN VQKDSIEEDL QLAMKGDAIH 720
ATSSSSISKL KKIVPLCGFS DPVYAEACIT NNQFDVVLDV LLVNQTKETL KNLHVQFATL 780
GDLKIIDTPQ KTNVIPHGFH KFTVTVKVSS ADTGVIFGNI IYDGAHGEDA RYVILNDVHV 840
DIMDYIKPAT ADDEHFRTMW NAFEWENKIS VKSQLPTLHA YLRELVKGTN MGILTPSESL 900
GEDDCRFLSC NLYAKSSFGE DALANLCIEK DSKTNDVIGY VRIRSKGQGL ALSLGDRVAL 960
IAKKTNKLAL THV 973 
Gene Ontology
 GO:0030126; C:COPI vesicle coat; IGI:SGD.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; IMP:SGD.
 GO:0006886; P:intracellular protein transport; IEA:InterPro. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR002553; Clathrin/coatomer_adapt-like_N.
 IPR011710; Coatomer_bsu_C.
 IPR016460; COPB1. 
Pfam
 PF01602; Adaptin_N
 PF07718; Coatamer_beta_C 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS