CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021697
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transmembrane 9 superfamily member 3 
Protein Synonyms/Alias
 EP70-P-iso; SM-11044-binding protein 
Gene Name
 TM9SF3 
Gene Synonyms/Alias
 SMBP; UNQ245/PRO282 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
59HNRQETYKYFSLPFCubiquitination[1, 2, 3]
71PFCVGSKKSISHYHEubiquitination[2]
160YYLWTYKKLEIGFNGubiquitination[2]
198MSYSVKWKKSDVKFEacetylation[4]
203KWKKSDVKFEDRFDKubiquitination[5]
210KFEDRFDKYLDPSFFubiquitination[1, 3]
257KDYARYSKEEEMDDMubiquitination[5, 6]
441PRPIPEKKWFMEPAVubiquitination[2]
587RKIYTNVKID*****ubiquitination[5, 6, 7]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
 CARBOHYD 174 174 N-linked (GlcNAc...).
 CARBOHYD 419 419 N-linked (GlcNAc...) (Potential).  
Keyword
 Complete proteome; Glycoprotein; Membrane; Reference proteome; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 589 AA 
Protein Sequence
MRPLPGALGV AAAAALWLLL LLLPRTRADE HEHTYQDKEE VVLWMNTVGP YHNRQETYKY 60
FSLPFCVGSK KSISHYHETL GEALQGVELE FSGLDIKFKD DVMPATYCEI DLDKEKRDAF 120
VYAIKNHYWY QMYIDDLPIW GIVGEADENG EDYYLWTYKK LEIGFNGNRI VDVNLTSEGK 180
VKLVPNTKIQ MSYSVKWKKS DVKFEDRFDK YLDPSFFQHR IHWFSIFNSF MMVIFLVGLV 240
SMILMRTLRK DYARYSKEEE MDDMDRDLGD EYGWKQVHGD VFRPSSHPLI FSSLIGSGCQ 300
IFAVSLIVII VAMIEDLYTE RGSMLSTAIF VYAATSPVNG YFGGSLYARQ GGRRWIKQMF 360
IGAFLIPAMV CGTAFFINFI AIYYHASRAI PFGTMVAVCC ICFFVILPLN LVGTILGRNL 420
SGQPNFPCRV NAVPRPIPEK KWFMEPAVIV CLGGILPFGS IFIEMYFIFT SFWAYKIYYV 480
YGFMMLVLVI LCIVTVCVTI VCTYFLLNAE DYRWQWTSFL SAASTAIYVY MYSFYYYFFK 540
TKMYGLFQTS FYFGYMAVFS TALGIMCGAI GYMGTSAFVR KIYTNVKID 589 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. 
Interpro
 IPR004240; EMP70. 
Pfam
 PF02990; EMP70 
SMART
  
PROSITE
  
PRINTS