CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002622
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mineralocorticoid receptor 
Protein Synonyms/Alias
 MR; Nuclear receptor subfamily 3 group C member 2 
Gene Name
 NR3C2 
Gene Synonyms/Alias
 MCR; MLR 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
89GILTSDIKTELESKEsumoylation[1, 2, 3]
399LNIVQYIKPEPDGAFsumoylation[1, 2, 3]
428SSFSVPIKQESTKHSsumoylation[1, 3]
494SGFPVGIKQEPDDGSsumoylation[1, 2]
953FRESHALKVEFPAMLsumoylation[1, 2]
Reference
 [1] Protein inhibitor of activated signal transducer and activator of transcription 1 interacts with the N-terminal domain of mineralocorticoid receptor and represses its transcriptional activity: implication of small ubiquitin-related modifier 1 modification.
 Tallec LP, Kirsh O, Lecomte MC, Viengchareun S, Zennaro MC, Dejean A, Lombès M.
 Mol Endocrinol. 2003 Dec;17(12):2529-42. [PMID: 14500761]
 [2] Coactivation of the N-terminal transactivation of mineralocorticoid receptor by Ubc9.
 Yokota K, Shibata H, Kurihara I, Kobayashi S, Suda N, Murai-Takeda A, Saito I, Kitagawa H, Kato S, Saruta T, Itoh H.
 J Biol Chem. 2007 Jan 19;282(3):1998-2010. [PMID: 17105732]
 [3] Sumoylation and proteasomal activity determine the transactivation properties of the mineralocorticoid receptor.
 Tirard M, Almeida OF, Hutzler P, Melchior F, Michaelidis TM.
 Mol Cell Endocrinol. 2007 Mar 30;268(1-2):20-9. [PMID: 17314004] 
Functional Description
 Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels. 
Sequence Annotation
 DNA_BIND 603 668 Nuclear receptor.
 ZN_FING 603 623 NR C4-type.
 ZN_FING 639 663 NR C4-type.
 REGION 1 602 Modulating.
 REGION 669 732 Hinge.
 REGION 733 984 Steroid-binding.
 REGION 782 785 Important for coactivator binding.
 BINDING 770 770 Steroid.
 BINDING 776 776 Steroid.
 BINDING 817 817 Steroid.
 BINDING 945 945 Steroid.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Disease mutation; DNA-binding; Endoplasmic reticulum; Lipid-binding; Membrane; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Receptor; Reference proteome; Steroid-binding; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 984 AA 
Protein Sequence
METKGYHSLP EGLDMERRWG QVSQAVERSS LGPTERTDEN NYMEIVNVSC VSGAIPNNST 60
QGSSKEKQEL LPCLQQDNNR PGILTSDIKT ELESKELSAT VAESMGLYMD SVRDADYSYE 120
QQNQQGSMSP AKIYQNVEQL VKFYKGNGHR PSTLSCVNTP LRSFMSDSGS SVNGGVMRAI 180
VKSPIMCHEK SPSVCSPLNM TSSVCSPAGI NSVSSTTASF GSFPVHSPIT QGTPLTCSPN 240
AENRGSRSHS PAHASNVGSP LSSPLSSMKS SISSPPSHCS VKSPVSSPNN VTLRSSVSSP 300
ANINNSRCSV SSPSNTNNRS TLSSPAASTV GSICSPVNNA FSYTASGTSA GSSTLRDVVP 360
SPDTQEKGAQ EVPFPKTEEV ESAISNGVTG QLNIVQYIKP EPDGAFSSSC LGGNSKINSD 420
SSFSVPIKQE STKHSCSGTS FKGNPTVNPF PFMDGSYFSF MDDKDYYSLS GILGPPVPGF 480
DGNCEGSGFP VGIKQEPDDG SYYPEASIPS SAIVGVNSGG QSFHYRIGAQ GTISLSRSAR 540
DQSFQHLSSF PPVNTLVESW KSHGDLSSRR SDGYPVLEYI PENVSSSTLR SVSTGSSRPS 600
KICLVCGDEA SGCHYGVVTC GSCKVFFKRA VEGQHNYLCA GRNDCIIDKI RRKNCPACRL 660
QKCLQAGMNL GARKSKKLGK LKGIHEEQPQ QQQPPPPPPP PQSPEEGTTY IAPAKEPSVN 720
TALVPQLSTI SRALTPSPVM VLENIEPEIV YAGYDSSKPD TAENLLSTLN RLAGKQMIQV 780
VKWAKVLPGF KNLPLEDQIT LIQYSWMCLS SFALSWRSYK HTNSQFLYFA PDLVFNEEKM 840
HQSAMYELCQ GMHQISLQFV RLQLTFEEYT IMKVLLLLST IPKDGLKSQA AFEEMRTNYI 900
KELRKMVTKC PNNSGQSWQR FYQLTKLLDS MHDLVSDLLE FCFYTFRESH ALKVEFPAML 960
VEIISDQLPK VESGNAKPLY FHRK 984 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0005496; F:steroid binding; IEA:UniProtKB-KW.
 GO:0003707; F:steroid hormone receptor activity; TAS:ProtInc.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006883; P:cellular sodium ion homeostasis; IEA:Compara.
 GO:0007588; P:excretion; IEA:Compara.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome. 
Interpro
 IPR008946; Nucl_hormone_rcpt_ligand-bd.
 IPR000536; Nucl_hrmn_rcpt_lig-bd_core.
 IPR001628; Znf_hrmn_rcpt.
 IPR013088; Znf_NHR/GATA. 
Pfam
 PF00104; Hormone_recep
 PF00105; zf-C4 
SMART
 SM00430; HOLI
 SM00399; ZnF_C4 
PROSITE
 PS00031; NUCLEAR_REC_DBD_1
 PS51030; NUCLEAR_REC_DBD_2 
PRINTS
 PR00047; STROIDFINGER.