CPLM-005054

Genbank Nucleotide ID

5'-3' exoribonuclease 1

Protein Synonyms/Alias

DNA strand transfer protein beta; STP-beta; KAR(-)-enhancing mutation protein; Strand exchange protein 1; p175

DST2; KEM1; RAR5; SEP1; SKI1; YGL173C; G1645

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Position	Peptide	Type	References
124	ENGDEIPKGEPFDSN	ubiquitination	[1]
192	IRHLKSQKDFNQNTR	ubiquitination	[1]
980	QMDHQAVKYYPSYIV	acetylation	[2]
1004	FLSKITSKFMITDAT	acetylation	[2]
1272	TAHKTPSKQQSEEKL	acetylation	[3]
1292	HDLLNFIKKDTNEKN	acetylation	[2]
1298	IKKDTNEKNSESVDN	ubiquitination	[1]
1494	GETKNSRKGEIKPSS	ubiquitination	[1]
1498	NSRKGEIKPSSGTNS	ubiquitination	[1]

[1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
[2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
[3] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591]

Functional Description

Multifunctional protein that exhibits several independent functions at different levels of the cellular processes. 5'-3' exonuclease component of the nonsense-mediated mRNA decay (NMD) which is a highly conserved mRNA degradation pathway, an RNA surveillance system whose role is to identify and rid cells of mRNA with premature termination codons and thus prevents accumulation of potentially harmful truncated proteins. The NMD pathway has a second role regulating the decay of wild- type mRNAs, and especially mRNAs that are important for telomere functions. Participate in CTH2-mediated and VTS1-mediated mRNA turnover. Involved in the degradation of several hypomodified mature tRNA species and participates in the 5'-processing or the degradation of the snoRNA precursors and rRNA processing. Involved in defense against virus and suppresses viral RNA recombination by rapidly removing the 5'-truncated RNAs, the substrates of recombination, and thus reducing the chance for recombination to occur in the parental strain. Required for the assembly of the virus-like particles of the Ty3 retrotransposon and contributes to the efficient generation of narnavirus 20S RNA by playing a major role in the elimination of the non-viral upstream sequences from the primary transcripts. Degrades single-stranded DNA (ss-DNA) and can renature complementary ss-DNA as well as catalyzes the formation of heteroduplex DNA from circular ss-DNA and homologous linear ds-DNA in vitro. Acts as a microtubule-associated protein which interacts with cytoplasmic microtubules through beta-tubulin and promotes in vitro assembly of tubulin into microtubules. Associates with microtubule functions such as chromosome transmission, nuclear migration, and SPB duplication. Has also a role in G1 to S transition and is involved in nuclear fusion during karyogamy. Required for the expression of ROK1 at the post- transcriptional level and for the alpha-factor induction of the karyogamy genes KAR3 and KAR4. Plays a role in filamentous growth.

MOD_RES 1506 1506 Phosphothreonine.
MOD_RES 1510 1510 Phosphoserine.

Complete proteome; Cytoplasm; Direct protein sequencing; Exonuclease; Hydrolase; Karyogamy; Magnesium; Microtubule; Nonsense-mediated mRNA decay; Nuclease; Phosphoprotein; Reference proteome; RNA-binding; rRNA processing.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0000932; C:cytoplasmic mRNA processing body; IDA:SGD.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0005874; C:microtubule; IEA:UniProtKB-KW.
GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
GO:0004534; F:5'-3' exoribonuclease activity; IDA:SGD.
GO:0004536; F:deoxyribonuclease activity; TAS:SGD.
GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
GO:0000741; P:karyogamy; IEA:UniProtKB-KW.
GO:0070651; P:nonfunctional rRNA decay; IMP:SGD.
GO:0000956; P:nuclear-transcribed mRNA catabolic process; TAS:SGD.
GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
GO:0006364; P:rRNA processing; TAS:SGD.
GO:0007089; P:traversing start control point of mitotic cell cycle; IMP:SGD.

IPR027073; 5_3_exoribonuclease.
IPR016494; 5_3_exoribonuclease_1.
IPR004859; Put_53exo.