CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012665
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription factor E2F4 
Protein Synonyms/Alias
 E2F-4 
Gene Name
 E2F4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
28SLGLLTTKFVSLLQEubiquitination[1]
37VSLLQEAKDGVLDLKubiquitination[1, 2]
73EGIGLIEKKSKNSIQubiquitination[1]
74GIGLIEKKSKNSIQWubiquitination[1]
82SKNSIQWKGVGPGCNubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. 
Sequence Annotation
 DNA_BIND 16 85 Potential.
 REGION 43 65 Leucine-zipper.
 REGION 86 181 Dimerization (Potential).
 REGION 337 413 Transactivation (Potential).
 REGION 390 407 Interaction with RBL1 and RBL2
 MOTIF 48 85 DEF box.
 MOTIF 389 392 HCFC1-binding-motif (HBM).
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 3D-structure; Acetylation; Activator; Cell cycle; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 413 AA 
Protein Sequence
MAEAGPQAPP PPGTPSRHEK SLGLLTTKFV SLLQEAKDGV LDLKLAADTL AVRQKRRIYD 60
ITNVLEGIGL IEKKSKNSIQ WKGVGPGCNT REIADKLIEL KAEIEELQQR EQELDQHKVW 120
VQQSIRNVTE DVQNSCLAYV THEDICRCFA GDTLLAIRAP SGTSLEVPIP EGLNGQKKYQ 180
IHLKSVSGPI EVLLVNKEAW SSPPVAVPVP PPEDLLQSPS AVSTPPPLPK PALAQSQEAS 240
RPNSPQLTPT AVPGSAEVQG MAGPAAEITV SGGPGTDSKD SGELSSLPLG PTTLDTRPLQ 300
SSALLDSSSS SSSSSSSSSN SNSSSSSGPN PSTSFEPIKA DPTGVLELPK ELSEIFDPTR 360
ECMSSELLEE LMSSEVFAPL LRLSPPPGDH DYIYNLDESE GVCDLFDVPV LNL 413 
Gene Ontology
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005667; C:transcription factor complex; IEA:InterPro.
 GO:0003677; F:DNA binding; IMP:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0008015; P:blood circulation; IEA:Compara.
 GO:0006884; P:cell volume homeostasis; IEA:Compara.
 GO:0042384; P:cilium assembly; IEA:Compara.
 GO:0002064; P:epithelial cell development; IEA:Compara.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0009887; P:organ morphogenesis; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; TAS:Reactome.
 GO:0042127; P:regulation of cell proliferation; IEA:Compara.
 GO:0008361; P:regulation of cell size; IEA:Compara.
 GO:0000083; P:regulation of transcription involved in G1/S phase of mitotic cell cycle; IEA:Compara.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
 GO:0007179; P:transforming growth factor beta receptor signaling pathway; TAS:Reactome. 
Interpro
 IPR015633; E2F.
 IPR003316; E2F_TDP.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF02319; E2F_TDP 
SMART
  
PROSITE
  
PRINTS