CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005983
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glucose-repressible alcohol dehydrogenase transcriptional effector 
Protein Synonyms/Alias
 Carbon catabolite repressor protein 4; Cytoplasmic deadenylase 
Gene Name
 CCR4 
Gene Synonyms/Alias
 YAL021C; FUN27 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
262TDSKTAKKQQPTGDSubiquitination[1]
307QALLQHKKLSQYSIDubiquitination[1]
613DQFKLITKDAMDFSGubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Acts as catalytic component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. CCR4 has 3'-5' RNase activity with a strong preference for polyadenylated substrates and also low exonuclease activity towards single stranded DNA. Discovered because of its role in the control of ADH2 gene expression. It is required for the expression of genes involved in non-fermentative growth and it mediates or is required for the action of the SPT6 and SPT10 genes. 
Sequence Annotation
 REPEAT 334 356 LRR 1.
 REPEAT 358 379 LRR 2.
 REPEAT 381 402 LRR 3.
 REPEAT 404 426 LRR 4.
 REPEAT 427 447 LRR 5.
 METAL 556 556 Magnesium.
 MOD_RES 33 33 Phosphothreonine.
 MOD_RES 278 278 Phosphoserine.
 MOD_RES 285 285 Phosphothreonine.  
Keyword
 3D-structure; Activator; Complete proteome; Cytoplasm; Exonuclease; Hydrolase; Leucine-rich repeat; Magnesium; Metal-binding; Nuclease; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; RNA-binding; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 837 AA 
Protein Sequence
MNDPSLLGYP NVGPQQQQQQ QQQQHAGLLG KGTPNALQQQ LHMNQLTGIP PPGLMNNSDV 60
HTSSNNNSRQ LLDQLANGNA NMLNMNMDNN NNNNNNNNNN NNNGGGSGVM MNASTAAVNS 120
IGMVPTVGTP VNINVNASNP LLHPHLDDPS LLNNPIWKLQ LHLAAVSAQS LGQPNIYARQ 180
NAMKKYLATQ QAQQAQQQAQ QQAQQQVPGP FGPGPQAAPP ALQPTDFQQS HIAEASKSLV 240
DCTKQALMEM ADTLTDSKTA KKQQPTGDST PSGTATNSAV STPLTPKIEL FANGKDEANQ 300
ALLQHKKLSQ YSIDEDDDIE NRMVMPKDSK YDDQLWHALD LSNLQIFNIS ANIFKYDFLT 360
RLYLNGNSLT ELPAEIKNLS NLRVLDLSHN RLTSLPAELG SCFQLKYFYF FDNMVTTLPW 420
EFGNLCNLQF LGVEGNPLEK QFLKILTEKS VTGLIFYLRD NRPEIPLPHE RRFIEINTDG 480
EPQREYDSLQ QSTEHLATDL AKRTFTVLSY NTLCQHYATP KMYRYTPSWA LSWDYRRNKL 540
KEQILSYDSD LLCLQEVESK TFEEYWVPLL DKHGYTGIFH AKARAKTMHS KDSKKVDGCC 600
IFFKRDQFKL ITKDAMDFSG AWMKHKKFQR TEDYLNRAMN KDNVALFLKL QHIPSGDTIW 660
AVTTHLHWDP KFNDVKTFQV GVLLDHLETL LKEETSHNFR QDIKKFPVLI CGDFNSYINS 720
AVYELINTGR VQIHQEGNGR DFGYMSEKNF SHNLALKSSY NCIGELPFTN FTPSFTDVID 780
YIWFSTHALR VRGLLGEVDP EYVSKFIGFP NDKFPSDHIP LLARFEFMKT NTGSKKV 837 
Gene Ontology
 GO:0030015; C:CCR4-NOT core complex; IPI:SGD.
 GO:0000932; C:cytoplasmic mRNA processing body; IDA:SGD.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0000175; F:3'-5'-exoribonuclease activity; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004535; F:poly(A)-specific ribonuclease activity; IEA:EC.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0000076; P:DNA replication checkpoint; IGI:SGD.
 GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IDA:SGD.
 GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IDA:SGD.
 GO:0048478; P:replication fork protection; IGI:SGD.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; IMP:SGD.
 GO:0007089; P:traversing start control point of mitotic cell cycle; IMP:SGD. 
Interpro
 IPR005135; Endo/exonuclease/phosphatase.
 IPR001611; Leu-rich_rpt.
 IPR025875; Leu-rich_rpt_4.
 IPR003591; Leu-rich_rpt_typical-subtyp. 
Pfam
 PF03372; Exo_endo_phos
 PF12799; LRR_4 
SMART
 SM00369; LRR_TYP 
PROSITE
 PS51450; LRR 
PRINTS