CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021840
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Formin-2 
Protein Synonyms/Alias
  
Gene Name
 Fmn2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
1444RKLKKDLKACEAEAGubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Plays a role in responses to DNA damage, cellular stress and hypoxia by protecting CDKN1A against degradation, and thereby plays a role in stress-induced cell cycle arrest. Protects cells against apoptosis by protecting CDKN1A against degradation (By similarity). Actin-binding protein that is involved in actin cytoskeleton assembly and reorganization. Acts as an actin nucleation factor and promotes assembly of actin filaments together with SPIRE1 and SPIRE2. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for asymmetric spindle positioning, asymmetric oocyte division and polar body extrusion during female germ cell meiosis. 
Sequence Annotation
 DOMAIN 735 1124 FH1.
 REPEAT 919 929 1.
 REPEAT 930 940 2.
 REPEAT 941 951 3.
 REPEAT 952 962 4.
 REPEAT 963 973 5.
 REPEAT 974 984 6.
 REPEAT 985 995 7.
 REPEAT 996 1006 8.
 REPEAT 1007 1017 9.
 REPEAT 1018 1028 10.
 REPEAT 1029 1039 11.
 REPEAT 1040 1050 12.
 DOMAIN 1139 1554 FH2.
 REGION 919 1039 12 X 11 AA tandem repeats of [MV]-G-I-P-
 REGION 1571 1578 Important for interaction with SPIRE1.
 MOD_RES 339 339 Phosphoserine.
 MOD_RES 493 493 Phosphoserine.
 MOD_RES 723 723 Phosphothreonine.
 MOD_RES 724 724 Phosphoserine.  
Keyword
 Actin-binding; Cell membrane; Coiled coil; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Developmental protein; DNA damage; Membrane; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Repeat; Stress response; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1578 AA 
Protein Sequence
MGNQDGKLKR SAGDASHEGG GAEDAAGPRD AEITKKASGS KKALGKHGKG GGGSGETSKK 60
KSKSDSRASV FSNLRIRKNL TKGKGACDSR EDVLDSQALP IGELDSAHSI VTKTPDLSLS 120
AEETGLSDTE CADPFEVIHP GASRPAEAGV GIQATAEDLE TAAGAQDGQR TSSGSDTDIY 180
SFHSATEQED LLSDIQQAIR LQQQQQQKLL LQDSEEPAAP PTAISPQPGA FLGLDQFLLG 240
PRSEAEKDTV QALPVRPDLP ETTKSLVPEH PPSSGSHLTS ETPGYATAPS AVTDSLSSPA 300
FTFPEAGPGE GAAGVPVAGT GDTDEECEED AFEDAPRGSP GEEWVPEVEE ASQRLEKEPE 360
EGMRESITSA VVSLPGSPAP SPRCFKPYPL ITPCYIKTTT RQLSSPNHSP SQSPNQSPRI 420
KKRPDPSVSR SSRTALASAA APAKKHRLEG GLTGGLSRSA DWTEELGVRT PGAGGSVHLL 480
GRGATADDSG GGSPVLAAKA PGAPATADGF QNVFTGRTLL EKLFSQQENG PPEEAEKFCS 540
RIIAMGLLLP FSDCFREPCN QNAGSSSAPF DQDQLYTWAA VSQPTHSMDY SEGQFPRREP 600
SMWPSSKLPE EEPSPKDVDT EPKSSILESP KKCSNGVQQE VFDVKSEGQA TVIQQLEQTI 660
EDLRTKIAEL EKQYPALDLE GPRGLSGLEN GLTASADVSL DALVLHGKVA QPPRTLEAKS 720
IQTSPTEEGR ILTLPPPKAP PEGLLGSPAA ASGESALLTS PSGPQTKFCS EISLIVSPRR 780
ISVQLDAQQI QSASQLPPPP PLLGSDSQGQ PSQPSLHTES ETSHEHSVSS SFGNNCNVPP 840
APPLPCTESS SFMPGLGMAI PPPPCLSDIT VPALPSPTAP ALQFSNLQGP EMLPAPPQPP 900
PLPGLGVPPP PPAPPLPGMG IPPPPPLPGM GIPPPPPLPG MGISPLPPLP GMGIPPPPPL 960
PGVGIPPPPP LPGVGIPPPP PLPGVGIPPP PPLPGVGIPP PPPLPGVGIP PPPPLPGVGI 1020
PPPPPLPGVG IPPPPPLPGV GIPPPPPLPG SGIPPPPALP GVAIPPPPPL PGMGVPPPAP 1080
PPPGAGIPPP PLLPGSGPPH SSQVGSSTLP AAPQGCGFLF PPLPTGLFGL GMNQDRVARK 1140
QLIEPCRPMK PLYWTRIQLH SKRDSSPSLI WEKIEEPSID CHEFEELFSK TAVKERKKPI 1200
SDTISKTKAK QVVKLLSNKR SQAVGILMSS LHLDMKDIQH AVVNLDNSVV DLETLQALYE 1260
NRAQSDELEK IEKHSRSSKD KENAKSLDKP EQFLYELSLI PNFSERVFCI LFQSTFSESI 1320
CSIRRKLELL QKLCETLKNG PGVMQVLGLV LAFGNYMNAG NKTRGQADGF GLDILPKLKD 1380
VKSSDNSRSL LSYIVSYYLR NFDEDAGKEQ CVFPLAEPQE LFQASQMKFE DFQKDLRKLK 1440
KDLKACEAEA GKVYQVSSAE HMQPFKENME QFISQAKIDQ ESQEAALTET HKCFLETTAY 1500
YFMKPKLGEK EVSPNVFFSV WHEFSSDFKD AWKKENKLIL QERVKEAEEV CRQKKGKSLY 1560
KVKPRHDSGI KAKISMKT 1578 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IEA:InterPro.
 GO:0005938; C:cell cortex; IDA:UniProtKB.
 GO:0030659; C:cytoplasmic vesicle membrane; IDA:UniProtKB.
 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0005730; C:nucleolus; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0003779; F:actin binding; ISS:UniProtKB.
 GO:0006915; P:apoptotic process; ISS:UniProtKB.
 GO:0071456; P:cellular response to hypoxia; ISS:UniProtKB.
 GO:0051295; P:establishment of meiotic spindle localization; IMP:UniProtKB.
 GO:0070649; P:formin-nucleated actin cable assembly; IMP:UniProtKB.
 GO:0051758; P:homologous chromosome movement towards spindle pole involved in homologous chromosome segregation; IMP:BHF-UCL.
 GO:0046907; P:intracellular transport; IMP:UniProtKB.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0042177; P:negative regulation of protein catabolic process; ISS:UniProtKB.
 GO:0048477; P:oogenesis; IMP:BHF-UCL.
 GO:0040038; P:polar body extrusion after meiotic divisions; IMP:BHF-UCL.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0006974; P:response to DNA damage stimulus; ISS:UniProtKB.
 GO:0016192; P:vesicle-mediated transport; IMP:UniProtKB. 
Interpro
 IPR003104; Actin-bd_FH2/DRF_autoreg.
 IPR015425; FH2_actin-bd.
 IPR001265; Formin.
 IPR009408; Formin_homology_1. 
Pfam
 PF06346; Drf_FH1
 PF02181; FH2 
SMART
 SM00498; FH2 
PROSITE
 PS51444; FH2 
PRINTS
 PR00828; FORMIN.