UniProt Accession

 VPS28_HUMAN; Q9UK41; Q86VK0 

Genbank Protein ID

 AF182844; AF205589; BC006485; BC019321; BC050713 

Genbank Nucleotide ID

 AAF00499.1; AAH06485.1; AAH19321.1; AAH50713.1 

Protein Name

 Vacuolar protein sorting-associated protein 28 homolog 

Protein Synonyms/Alias

 H-Vps28; ESCRT-I complex subunit VPS28 

Gene Name

 VPS28 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
17	GIGAPGNKPELYEEV	ubiquitination	[1]
25	PELYEEVKLYKNARE	ubiquitination	[1]
28	YEEVKLYKNAREREK	ubiquitination	[1]
58	ALEKAYIKDCVSPSE	ubiquitination	[1, 2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Component of the ESCRT-I complex, a regulator of vesicular trafficking process. 

Sequence Annotation

 DOMAIN 13 120 VPS28 N-terminal.
 DOMAIN 124 220 VPS28 C-terminal.  

Keyword

 Alternative splicing; Cell membrane; Complete proteome; Endosome; Membrane; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 221 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:MGI.
 GO:0000813; C:ESCRT I complex; IDA:UniProtKB.
 GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0046788; P:egress of virus within host cell; TAS:Reactome.
 GO:0016197; P:endosomal transport; TAS:Reactome.
 GO:0031397; P:negative regulation of protein ubiquitination; IDA:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IMP:UniProtKB.
 GO:0019082; P:viral protein processing; TAS:Reactome.
 GO:0019068; P:virion assembly; TAS:Reactome. 

Interpro

 IPR007143; VPS28.
 IPR017899; VPS28_C.
 IPR017898; VPS28_N. 

Pfam

 PF03997; VPS28 

SMART

  

PROSITE

 PS51310; VPS28_C
 PS51313; VPS28_N 

PRINTS