UniProt Accession

 UBC4_YEAST; P15731; D6VQ81 

Genbank Protein ID

 X17493; X76294; Z35951; BK006936 

Genbank Nucleotide ID

 CAA35528.1; CAA53942.1; CAA85027.1; DAA07201.1 

Protein Name

 Ubiquitin-conjugating enzyme E2 4 

Protein Synonyms/Alias

 Ubiquitin carrier protein 4; Ubiquitin-protein ligase 4 

Gene Name

 UBC4 

Gene Synonyms/Alias

 YBR082C; YBR0745 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
9	SSSKRIAKELSDLER	acetylation	[1, 2]
91	NICLDILKDQWSPAL	ubiquitination	[3, 4]
134	IYKTDRPKYEATARE	acetylation	[2]
134	IYKTDRPKYEATARE	ubiquitination	[4]

Reference

 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [4] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Mediates ubiquitination of PEX5. 

Sequence Annotation

 ACT_SITE 86 86 Glycyl thioester intermediate.
 MOD_RES 12 12 Phosphoserine.  

Keyword

 3D-structure; ATP-binding; Complete proteome; Direct protein sequencing; Ligase; Nucleotide-binding; Phosphoprotein; Reference proteome; Stress response; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 148 AA 

Protein Sequence

Gene Ontology

 GO:0000502; C:proteasome complex; IPI:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0043130; F:ubiquitin binding; IDA:SGD.
 GO:0004842; F:ubiquitin-protein ligase activity; IMP:UniProtKB.
 GO:0006513; P:protein monoubiquitination; IDA:SGD.
 GO:0000209; P:protein polyubiquitination; IMP:UniProtKB.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW.
 GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IMP:SGD. 

Interpro

 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS