UniProt Accession

 VAMP8_MOUSE; O70404; Q9CRA3 

Genbank Protein ID

 AF053724; AF045661; AF247556; AK002268; AK013279; BC012668 

Genbank Nucleotide ID

 AAC06371.1; AAC23665.1; AAK48423.1; BAB21977.1; BAB28766.1; AAH12668.1 

Protein Name

 Vesicle-associated membrane protein 8 

Protein Synonyms/Alias

 VAMP-8; Endobrevin; Edb 

Gene Name

 Vamp8 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
24	QSEVEGVKNIMTQNV	ubiquitination	[1]
47	NLDHLRNKTEDLEAT	ubiquitination	[1]
59	EATSEHFKTTSQKVA	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 SNAREs, Soluble N-ethylmaleimide-sensitive factor- attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Also required for dense-granule secretion in platelets. Plays also a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes. 

Sequence Annotation

 DOMAIN 12 72 v-SNARE coiled-coil homology.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 18 18 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Autophagy; Coiled coil; Complete proteome; Endosome; Lysosome; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transmembrane; Transmembrane helix; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 101 AA 

Protein Sequence

Gene Ontology

 GO:0005769; C:early endosome; TAS:MGI.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0031902; C:late endosome membrane; ISS:UniProtKB.
 GO:0005765; C:lysosomal membrane; ISS:UniProtKB.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0055037; C:recycling endosome; IEA:Compara.
 GO:0030141; C:secretory granule; IDA:MGI.
 GO:0031201; C:SNARE complex; ISS:UniProtKB.
 GO:0000046; P:autophagic vacuole fusion; ISS:UniProtKB.
 GO:0006461; P:protein complex assembly; IEA:Compara.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0030100; P:regulation of endocytosis; IMP:MGI.
 GO:0006906; P:vesicle fusion; IEA:Compara. 

Interpro

 IPR001388; Synaptobrevin.
 IPR016444; Synaptobrevin_met/fun. 

Pfam

 PF00957; Synaptobrevin 

SMART

  

PROSITE

 PS00417; SYNAPTOBREVIN
 PS50892; V_SNARE 

PRINTS

 PR00219; SYNAPTOBREVN.