CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030737
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear receptor coactivator 4 
Protein Synonyms/Alias
 cDNA FLJ59491, highly similar to Nuclear receptor coactivator 4 
Gene Name
 NCOA4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
31RLGSLTLKPEDSTVLubiquitination[1]
81NIGPFLEKRGCISMPubiquitination[1, 2, 3, 4]
131PQDWLTQKQTLENSQubiquitination[5]
163GLENWLLKSEKSSYQubiquitination[1, 2, 3, 5]
171SEKSSYQKCNSHSTTubiquitination[1, 3]
213VTPQESHKLRKPENGubiquitination[1, 2, 3, 5]
256NCQGNQPKGVEIENLubiquitination[1, 3]
267IENLGNLKCLNDHLEubiquitination[1, 3, 4, 6]
276LNDHLEAKKPLSTPSubiquitination[1]
277NDHLEAKKPLSTPSMubiquitination[3]
324VCDENCEKEALYKWLubiquitination[1, 3, 4, 6]
329CEKEALYKWLLKKEGubiquitination[3, 7]
339LKKEGKDKNGMPVEPubiquitination[5]
365NMWLCPRKEVIEQTKubiquitination[3]
372KEVIEQTKAPKAMTPubiquitination[1, 2, 3, 4, 5]
375IEQTKAPKAMTPSRIubiquitination[3]
390ADSFQVIKNSPLSEWubiquitination[1, 2, 3]
404WLIRPPYKEGSPKEVubiquitination[3]
409PYKEGSPKEVPGTEDubiquitination[3]
444ADWVLPGKKMGNLSQubiquitination[1, 3]
445DWVLPGKKMGNLSQLubiquitination[3]
458QLSSGEDKWLLRKKAubiquitination[2, 3]
503CMQLKVDKEKWLYRTubiquitination[3]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Receptor; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 514 AA 
Protein Sequence
MNRWTLFISL KRRHFNSRLS SSTRLGSLTL KPEDSTVLLF EADTITLRQT ITTFGSLKTI 60
QIPEHLMAHA SSANIGPFLE KRGCISMPEQ KSASGIVAVP FSEWLLGSKP ASGYQAPYIP 120
STDPQDWLTQ KQTLENSQTS SRACNFFNNV GGNLKGLENW LLKSEKSSYQ KCNSHSTTSS 180
FSIEMEKVGD QELPDQDEMD LSDWLVTPQE SHKLRKPENG SRETSEKFKL LFQSYNVNDW 240
LVKTDSCTNC QGNQPKGVEI ENLGNLKCLN DHLEAKKPLS TPSMVTEDWL VQNHQDPCKV 300
EEVCRANEPC TSFAECVCDE NCEKEALYKW LLKKEGKDKN GMPVEPKPEP EKHKDSLNMW 360
LCPRKEVIEQ TKAPKAMTPS RIADSFQVIK NSPLSEWLIR PPYKEGSPKE VPGTEDRAGK 420
QKFKSPMNTS WCSFNTADWV LPGKKMGNLS QLSSGEDKWL LRKKAQEVLL NSPLQEEHNF 480
PPDHYGLPAV CDLFACMQLK VDKEKWLYRT PLQM 514 
Gene Ontology
  
Interpro
 IPR022174; ARA70. 
Pfam
 PF12489; ARA70 
SMART
  
PROSITE
  
PRINTS