CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018799
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA repair and recombination protein RAD54-like 
Protein Synonyms/Alias
 RAD54 homolog; hHR54; hRAD54 
Gene Name
 RAD54L 
Gene Synonyms/Alias
 RAD54A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
53CFLSPFRKPLSQLTNubiquitination[1]
80FIRSILSKPFKVPIPubiquitination[1]
208LRQSPECKPEIDKAVubiquitination[1]
224VSPSSLVKNWYNEVGubiquitination[1]
232NWYNEVGKWLGGRIQubiquitination[1]
248LAIDGGSKDEIDQKLubiquitination[1]
254SKDEIDQKLEGFMNQubiquitination[1]
302CDEGHRLKNSENQTYubiquitination[1, 2, 3]
355GTAHEFKKHFELPILubiquitination[1]
363HFELPILKGRDAAASubiquitination[1]
402RTSDILSKYLPVKIEubiquitination[1]
407LSKYLPVKIEQVVCCubiquitination[1]
425PLQTELYKRFLRQAKubiquitination[1]
441AEELLEGKMSVSSLSubiquitination[1]
550LDGTMSIKKRAKVVEubiquitination[1]
630SAGTIEEKIFQRQSHubiquitination[1, 3]
639FQRQSHKKALSSCVVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (By similarity). May play also an essential role in telomere length maintenance and telomere capping in mammalian cells. 
Sequence Annotation
 DOMAIN 170 345 Helicase ATP-binding.
 DOMAIN 500 653 Helicase C-terminal.
 NP_BIND 183 190 ATP (Potential).
 REGION 2 9 Required for chromatin remodeling, strand
 MOTIF 296 299 DEGH box.  
Keyword
 ATP-binding; Complete proteome; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 747 AA 
Protein Sequence
MRRSLAPSQL AKRKPEGRSC DDEDWQPGLV TPRKRKSSSE TQIQECFLSP FRKPLSQLTN 60
QPPCLDSSQH EAFIRSILSK PFKVPIPNYQ GPLGSRALGL KRAGVRRALH DPLEKDALVL 120
YEPPPLSAHD QLKLDKEKLP VHVVVDPILS KVLRPHQREG VKFLWECVTS RRIPGSHGCI 180
MADEMGLGKT LQCITLMWTL LRQSPECKPE IDKAVVVSPS SLVKNWYNEV GKWLGGRIQP 240
LAIDGGSKDE IDQKLEGFMN QRGARVSSPI LIISYETFRL HVGVLQKGSV GLVICDEGHR 300
LKNSENQTYQ ALDSLNTSRR VLISGTPIQN DLLEYFSLVH FVNSGILGTA HEFKKHFELP 360
ILKGRDAAAS EADRQLGEER LRELTSIVNR CLIRRTSDIL SKYLPVKIEQ VVCCRLTPLQ 420
TELYKRFLRQ AKPAEELLEG KMSVSSLSSI TSLKKLCNHP ALIYDKCVEE EDGFVGALDL 480
FPPGYSSKAL EPQLSGKMLV LDYILAVTRS RSSDKVVLVS NYTQTLDLFE KLCRARRYLY 540
VRLDGTMSIK KRAKVVERFN SPSSPDFVFM LSSKAGGCGL NLIGANRLVM FDPDWNPAND 600
EQAMARVWRD GQKKTCYIYR LLSAGTIEEK IFQRQSHKKA LSSCVVDEEQ DVERHFSLGE 660
LKELFILDEA SLSDTHDRLH CRRCVNSRQI RPPPDGSDCT SDLAGWNHCT DKWGLRDEVL 720
QAAWDAASTA ITFVFHQRSH EEQRGLR 747 
Gene Ontology
 GO:0005634; C:nucleus; TAS:ProtInc.
 GO:0036310; F:annealing helicase activity; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
 GO:0051276; P:chromosome organization; IEA:Compara.
 GO:0006310; P:DNA recombination; TAS:ProtInc.
 GO:0006281; P:DNA repair; TAS:ProtInc.
 GO:0000724; P:double-strand break repair via homologous recombination; IEA:Compara.
 GO:0007126; P:meiosis; TAS:ProtInc.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0010212; P:response to ionizing radiation; IEA:Compara. 
Interpro
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR013967; Rad54_N.
 IPR000330; SNF2_N. 
Pfam
 PF00271; Helicase_C
 PF08658; Rad54_N
 PF00176; SNF2_N 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS