CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037265
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Pyruvate dehydrogenase protein X component, mitochondrial 
Protein Synonyms/Alias
  
Gene Name
 PDHX 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
105IVVEEGSKNIRLGSLacetylation[1, 2]
179AARNILEKHSLDASQacetylation[1, 2, 3, 4]
310DLVKDDIKVSVNDFIacetylation[2]
379QEIADSVKALSKKARacetylation[2]
445EDEEGNAKLQQRQLIubiquitination[5]
476TRFLKSFKANLENPIacetylation[4]
Reference
 [1] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
  
Keyword
 Acyltransferase; Complete proteome; Lipoyl; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 486 AA 
Protein Sequence
MQSGGAEGSP GAGRTGRGPG SGKAPPAEIS SGAPDFPGGD PIKILMPSLS PTMEEGNIVK 60
WLKKEGEAVS AGDALCEIET DKAVVTLDAS DDGILAKIVV EEGSKNIRLG SLIGLIVEEG 120
EDWKHVEIPK DVGPPPPVSK PSEPRPSPEP QISIPVKKEH IPGTLRFRLS PAARNILEKH 180
SLDASQGTAT GPRGIFTKED ALKLVQLKQT GKITESRPTP APTATPTAPS PLQATAGPSY 240
PRPVIPPVST PGQPNAVGTF TEIPASNIRR VIAKRLTESK STVPHAYATA DCDLGAVLKV 300
RQDLVKDDIK VSVNDFIIKA AAVTLKQMPD VNVSWDGEGP KQLPFIDISV AVATDKGLLT 360
PIIKDAAAKG IQEIADSVKA LSKKARDGKL LPEEYQGGSF SISNLGMFGI DEFTAVINPP 420
QACILAVGRF RPVLKLTEDE EGNAKLQQRQ LITVTMSSDS RVVDDELATR FLKSFKANLE 480
NPIRLA 486 
Gene Ontology
 GO:0016746; F:transferase activity, transferring acyl groups; IEA:UniProtKB-KW. 
Interpro
 IPR003016; 2-oxoA_DH_lipoyl-BS.
 IPR001078; 2-oxoacid_DH_actylTfrase.
 IPR000089; Biotin_lipoyl.
 IPR023213; CAT-like_dom.
 IPR004167; E3-bd.
 IPR011053; Single_hybrid_motif. 
Pfam
 PF00198; 2-oxoacid_dh
 PF00364; Biotin_lipoyl
 PF02817; E3_binding 
SMART
  
PROSITE
 PS50968; BIOTINYL_LIPOYL
 PS00189; LIPOYL 
PRINTS