CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023943
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sorting nexin-5 
Protein Synonyms/Alias
  
Gene Name
 SNX5 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
118EGEGSMTKEEFAKMKubiquitination[1, 2]
180SVRRKNTKEMFGGFFubiquitination[2, 3]
188EMFGGFFKSVVKSADubiquitination[2, 3, 4]
192GFFKSVVKSADEVLFubiquitination[1]
203EVLFTGVKEVDDFFEubiquitination[1]
263LEEPTVIKKYLLKVAubiquitination[1]
268VIKKYLLKVAELFEKubiquitination[2]
275KVAELFEKLRKVEGRacetylation[5]
275KVAELFEKLRKVEGRubiquitination[1, 2]
290VSSDEDLKLTELLRYubiquitination[1, 2]
314DLLYRRTKALIDYENubiquitination[1]
324IDYENSNKALDKARLubiquitination[2]
337RLKSKDVKLAEAHQQacetylation[6]
337RLKSKDVKLAEAHQQubiquitination[2, 6]
365KEELINFKRKRVAAFubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 May be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation. 
Sequence Annotation
 DOMAIN 25 172 PX.
 DOMAIN 202 404 BAR.
 REGION 40 46 Phosphatidylinositol bisphosphate binding
 REGION 99 105 Phosphatidylinositol bisphosphate binding
 REGION 113 116 Phosphatidylinositol bisphosphate binding
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 275 275 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Endocytosis; Endosome; Lipid-binding; Membrane; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 404 AA 
Protein Sequence
MAAVPELLQQ QEEDRSKLRS VSVDLNVDPS LQIDIPDALS ERDKVKFTVH TKTTLPTFQS 60
PEFSVTRQHE DFVWLHDTLI ETTDYAGLII PPAPTKPDFD GPREKMQKLG EGEGSMTKEE 120
FAKMKQELEA EYLAVFKKTV SSHEVFLQRL SSHPVLSKDR NFHVFLEYDQ DLSVRRKNTK 180
EMFGGFFKSV VKSADEVLFT GVKEVDDFFE QEKNFLINYY NRIKDSCVKA DKMTRSHKNV 240
ADDYIHTAAC LHSLALEEPT VIKKYLLKVA ELFEKLRKVE GRVSSDEDLK LTELLRYYML 300
NIEAAKDLLY RRTKALIDYE NSNKALDKAR LKSKDVKLAE AHQQECCQKF EQLSESAKEE 360
LINFKRKRVA AFRKNLIEMS ELEIKHARNN VSLLQSCIDL FKNN 404 
Gene Ontology
 GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0031313; C:extrinsic to endosome membrane; IDA:UniProtKB.
 GO:0031234; C:extrinsic to internal side of plasma membrane; IDA:UniProtKB.
 GO:0070685; C:macropinocytic cup; IDA:UniProtKB.
 GO:0001891; C:phagocytic cup; IEA:UniProtKB-SubCell.
 GO:0001726; C:ruffle; IEA:UniProtKB-SubCell.
 GO:0035091; F:phosphatidylinositol binding; IDA:UniProtKB.
 GO:0007154; P:cell communication; IEA:InterPro.
 GO:0006907; P:pinocytosis; IDA:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 
Interpro
 IPR001683; Phox.
 IPR014637; Snx5_Snx6.
 IPR015404; Vps5_C. 
Pfam
 PF00787; PX
 PF09325; Vps5 
SMART
 SM00312; PX 
PROSITE
 PS51021; BAR
 PS50195; PX 
PRINTS