UniProt Accession

 PAXB1_HUMAN; Q9Y5B6; D3DSE7; Q96DU8; Q9NYQ0 

Genbank Protein ID

 AY033903; AY033904; AY033905; AY033906; AJ279080; AJ279081; CH471079; CH471079; AF231920; AF153208 

Genbank Nucleotide ID

 AAK68721.1; AAK68722.1; AAK68723.1; AAK68724.1; CAC40813.1; CAC40814.1; EAX09859.1; EAX09861.1; AAF72944.1; AAD34617.1 

Protein Name

 PAX3- and PAX7-binding protein 1 

Protein Synonyms/Alias

 GC-rich sequence DNA-binding factor 1 

Gene Name

 PAXBP1 

Gene Synonyms/Alias

 C21orf66; GCFC; GCFC1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
164	ESEQPLDKTGHVKDT	acetylation	[1]
278	RRIVFSVKEKSQRQK	acetylation	[1, 2, 3]
442	GGIGERYKFLQEMRG	ubiquitination	[4]
682	LLPTIVEKVILPKLT	ubiquitination	[5, 6]

Reference

 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Adapter protein linking the transcription factors PAX3 and PAX7 to the histone methylation machinery and involved in myogenesis. Associates with an histone methyltransferase complex that specifically mediates dimethylation and trimethylation of 'Lys-4' of histone H3. Mediates the recruitment of that complex to the transcription factors PAX3 and PAX7 on chromatin to regulate the expression of genes involved in muscle progenitor cells proliferation including ID3 and CDC20 (By similarity). 

Sequence Annotation

 REGION 378 558 Necessary and sufficient for interaction
 MOD_RES 16 16 Phosphoserine.
 MOD_RES 154 154 Phosphoserine.
 MOD_RES 155 155 Phosphoserine.
 MOD_RES 158 158 Phosphoserine.
 MOD_RES 262 262 Phosphoserine.
 MOD_RES 295 295 Phosphoserine.
 MOD_RES 557 557 Phosphoserine.
 MOD_RES 558 558 Phosphoserine.  

Keyword

 Alternative splicing; Complete proteome; DNA-binding; Myogenesis; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 917 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; NAS:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0007517; P:muscle organ development; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; NAS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR012890; GCFC.
 IPR022783; GCFC_dom. 

Pfam

 PF07842; GCFC 

SMART

  

PROSITE

  

PRINTS