UniProt Accession

 B4DZU0_HUMAN; B4DZU0 

Genbank Protein ID

 AC096637; AL356310; AK303092 

Genbank Nucleotide ID

 BAG64202.1 

Protein Name

 Serine/threonine-protein kinase Nek2 

Protein Synonyms/Alias

 cDNA FLJ53945, highly similar to Serine/threonine-protein kinase Nek2 (EC 2.7.11.1) 

Gene Name

 NEK2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
20	VNLLRELKHPNIVRY	ubiquitination	[1]
100	TVLHRDLKPANVFLD	ubiquitination	[1, 2]
109	ANVFLDGKQNVKLGD	ubiquitination	[1, 2, 3]
113	LDGKQNVKLGDFGLA	ubiquitination	[1, 2, 3]
131	NHDTSFAKTFVGTPY	ubiquitination	[1, 3]
184	SQKELAGKIREGKFR	ubiquitination	[1]
252	RQLGEPEKSQDSSPV	ubiquitination	[1]
266	VLSELKLKEIQLQER	ubiquitination	[1]
287	REERLEQKEQELCVR	ubiquitination	[1]
301	RERLAEDKLARAENL	ubiquitination	[1]
310	ARAENLLKNYSLLKE	ubiquitination	[1, 3]
316	LKNYSLLKERKFLSL	ubiquitination	[1, 3]
319	YSLLKERKFLSLASN	ubiquitination	[1, 4]
338	NLPSSVIKKKVHFSG	ubiquitination	[3, 5]
339	LPSSVIKKKVHFSGE	ubiquitination	[1]
348	VHFSGESKENIMRSE	ubiquitination	[1, 2, 3, 5, 6]
364	SESQLTSKSKCKDLK	ubiquitination	[1, 2, 3, 5]
394	IEKNYQLKSRQILGM	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; Kinase; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 402 AA 

Protein Sequence

Gene Ontology

 GO:0000794; C:condensed nuclear chromosome; IEA:Compara.
 GO:0030496; C:midbody; IEA:Compara.
 GO:0000922; C:spindle pole; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:InterPro.
 GO:0001824; P:blastocyst development; IEA:Compara.
 GO:0000070; P:mitotic sister chromatid segregation; IEA:Compara.
 GO:0043392; P:negative regulation of DNA binding; IEA:Compara.
 GO:0090307; P:spindle assembly involved in mitosis; IEA:Compara. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

  

PROSITE

 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS