CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024174
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 26S proteasome non-ATPase regulatory subunit 10 
Protein Synonyms/Alias
 26S proteasome regulatory subunit p28; Gankyrin 
Gene Name
 Psmd10 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
30KERILADKSLATRTDubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module (By similarity). Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pahway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis. 
Sequence Annotation
 REPEAT 3 36 ANK 1.
 REPEAT 37 69 ANK 2.
 REPEAT 70 102 ANK 3.
 REPEAT 103 135 ANK 4.
 REPEAT 136 168 ANK 5.
 REPEAT 169 201 ANK 6.
 REPEAT 202 226 ANK 7.  
Keyword
 3D-structure; ANK repeat; Apoptosis; Chaperone; Complete proteome; Cytoplasm; Nucleus; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 231 AA 
Protein Sequence
MEGCVSNIMI CNLAYSGKLD ELKERILADK SLATRTDQDS RTALHWACSA GHTEIVEFLL 60
QLGVPVNDKD DAGWSPLHIA ASAGRDEIVK ALLVKGAHVN AVNQNGCTPL HYAASKNRHE 120
IAVMLLEGGA NPDAKDHYDA TAMHRAAAKG NLKMVHILLF YKASTNIQDT EGNTPLHLAC 180
DEERVEEAKF LVTQGASIYI ENKEEKTPLQ VAKGGLGLIL KRLAESEEAS M 231 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0007253; P:cytoplasmic sequestering of NF-kappaB; ISS:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; ISS:UniProtKB.
 GO:0043518; P:negative regulation of DNA damage response, signal transduction by p53 class mediator; ISS:UniProtKB.
 GO:0043409; P:negative regulation of MAPK cascade; ISS:UniProtKB.
 GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; ISS:UniProtKB.
 GO:0090201; P:negative regulation of release of cytochrome c from mitochondria; ISS:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0030307; P:positive regulation of cell growth; ISS:UniProtKB.
 GO:0045737; P:positive regulation of cyclin-dependent protein kinase activity; ISS:UniProtKB.
 GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
 GO:0031398; P:positive regulation of protein ubiquitination; ISS:UniProtKB.
 GO:0070682; P:proteasome regulatory particle assembly; ISS:UniProtKB. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom. 
Pfam
 PF00023; Ank
 PF12796; Ank_2 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT 
PRINTS
 PR01415; ANKYRIN.