CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031404
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleoporin NUP53 
Protein Synonyms/Alias
 cDNA FLJ57668, highly similar to Nucleoporin NUP53 
Gene Name
 NUP35 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
66QPVVPAHKDKSGAPPubiquitination[1, 2, 3]
68VVPAHKDKSGAPPVRubiquitination[2, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 309 AA 
Protein Sequence
MMLGSPTSPK PGVNAQFLPG FLMGDLPAPV TPQPRSISGP SVGVMEMRSP LLAGGSPPQP 60
VVPAHKDKSG APPVRSIYDD ISSPGLGSTP LTSRRQPNIS VMQSPLVGVT STPGTGQSMF 120
SPASIGQPRK TTLSPAQLDP FYTQGDSLTS EDHLDDSWVT VFGFPQASAS YILLQFAQYG 180
NILKHVMSNT GNWMHIRYQS KLQARKALSK DGRIFGESIM IGVKPCIDKS VMESSDRCAL 240
SSPSLAFTPP IKTLGTPTQP GSTPRISTMR PLATAYKAST SDYQVISDRQ TPKKDESLVS 300
KAMEYMFGW 309 
Gene Ontology
 GO:0045111; C:intermediate filament cytoskeleton; IDA:HPA.
 GO:0031965; C:nuclear membrane; IDA:HPA.
 GO:0055085; P:transmembrane transport; IEA:InterPro. 
Interpro
 IPR017389; Nucleoporin_NUP53.
 IPR007846; RRM_NUP35_dom. 
Pfam
 PF05172; Nup35_RRM 
SMART
  
PROSITE
 PS51472; RRM_NUP35 
PRINTS