CPLM-011381

Genbank Nucleotide ID

Exosome complex component RRP45

Protein Synonyms/Alias

Autoantigen PM/Scl 1; Exosome component 9; P75 polymyositis-scleroderma overlap syndrome-associated autoantigen; Polymyositis/scleroderma autoantigen 1; Polymyositis/scleroderma autoantigen 75 kDa; PM/Scl-75

Homo sapiens (Human)

Position	Peptide	Type	References
2	******MKETPLSNC	ubiquitination	[1]
52	CCIVELGKTRVLGQV	ubiquitination	[1, 2]
67	SCELVSPKLNRATEG	ubiquitination	[1]
101	RQSDLLVKLNRLMER	ubiquitination	[1]
114	ERCLRNSKCIDTESL	ubiquitination	[1]
257	CSKIAGVKVAEITEL	ubiquitination	[1]
267	EITELILKALENDQK	ubiquitination	[1]
274	KALENDQKVRKEGGK	ubiquitination	[1]
277	ENDQKVRKEGGKFGF	ubiquitination	[1]
281	KVRKEGGKFGFAESI	ubiquitination	[1, 2]
297	NQRITAFKMEKAPID	ubiquitination	[1]
300	ITAFKMEKAPIDTSD	ubiquitination	[1]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]

Functional Description

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC9 binds to ARE-containing RNAs.

REGION 1 268 ARE binding.
MOD_RES 297 297 N6-acetyllysine.
MOD_RES 306 306 Phosphoserine.
MOD_RES 392 392 Phosphoserine.
MOD_RES 394 394 Phosphoserine.

3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Exosome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; RNA-binding; rRNA processing.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0000176; C:nuclear exosome (RNase complex); IDA:UniProtKB.
GO:0005730; C:nucleolus; IDA:UniProtKB.
GO:0005634; C:nucleus; IDA:UniProtKB.
GO:0000175; F:3'-5'-exoribonuclease activity; NAS:UniProtKB.
GO:0017091; F:AU-rich element binding; IDA:UniProtKB.
GO:0043928; P:exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay; IMP:UniProtKB.
GO:0006955; P:immune response; NAS:UniProtKB.
GO:0071028; P:nuclear mRNA surveillance; IMP:UniProtKB.
GO:0071035; P:nuclear polyadenylation-dependent rRNA catabolic process; IMP:UniProtKB.
GO:0030307; P:positive regulation of cell growth; IMP:UniProtKB.
GO:0006364; P:rRNA processing; NAS:UniProtKB.

IPR001247; ExoRNase_PH_dom1.
IPR015847; ExoRNase_PH_dom2.
IPR020568; Ribosomal_S5_D2-typ_fold.

PF01138; RNase_PH
PF03725; RNase_PH_C