UniProt Accession

 ADH2_YEAST; P00331; D6W0D0 

Genbank Protein ID

 J01314; M38457; Z49212; BK006946 

Genbank Nucleotide ID

 AAA34408.1; AAA34411.1; CAA89136.1; DAA10204.1 

Protein Name

 Alcohol dehydrogenase 2 

Protein Synonyms/Alias

 Alcohol dehydrogenase II; YADH-2 

Gene Name

 ADH2 

Gene Synonyms/Alias

 ADR2; YMR303C; YM9952.05C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
60	GDWPLPTKLPLVGGH	ubiquitination	[1]
81	VGMGENVKGWKIGDY	ubiquitination	[2]
332	SLPEIYEKMEKGQIA	acetylation	[3]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 This isozyme preferentially catalyzes the conversion of ethanol to acetaldehyde. Acts on a variety of primary unbranched aliphatic alcohols. 

Sequence Annotation

 NP_BIND 178 184 NAD (By similarity).
 NP_BIND 269 271 NAD (By similarity).
 METAL 44 44 Zinc 1; catalytic.
 METAL 67 67 Zinc 1; catalytic.
 METAL 98 98 Zinc 2.
 METAL 101 101 Zinc 2.
 METAL 104 104 Zinc 2.
 METAL 112 112 Zinc 2.
 METAL 154 154 Zinc 1; catalytic.
 BINDING 202 202 NAD (By similarity).
 BINDING 207 207 NAD (By similarity).
 BINDING 341 341 NAD (By similarity).
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 213 213 Phosphoserine (By similarity).
 MOD_RES 223 223 Phosphothreonine (By similarity).
 MOD_RES 279 279 Phosphoserine (By similarity).
 MOD_RES 316 316 Phosphoserine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Metal-binding; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 348 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0004022; F:alcohol dehydrogenase (NAD) activity; IDA:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0000947; P:amino acid catabolic process to alcohol via Ehrlich pathway; IGI:SGD.
 GO:0006067; P:ethanol metabolic process; IDA:SGD.
 GO:0006116; P:NADH oxidation; IDA:SGD. 

Interpro

 IPR013149; ADH_C.
 IPR013154; ADH_GroES-like.
 IPR002085; ADH_SF_Zn-type.
 IPR002328; ADH_Zn_CS.
 IPR011032; GroES-like.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF08240; ADH_N
 PF00107; ADH_zinc_N 

SMART

  

PROSITE

 PS00059; ADH_ZINC 

PRINTS