CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000045
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Membrane-associated progesterone receptor component 1 
Protein Synonyms/Alias
 mPR 
Gene Name
 PGRMC1 
Gene Synonyms/Alias
 HPR6.6; PGRMC 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
96ILMAINGKVFDVTKGubiquitination[1, 2, 3, 4]
102GKVFDVTKGRKFYGPubiquitination[1, 4, 5]
105FDVTKGRKFYGPEGPubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 9]
132LATFCLDKEALKDEYubiquitination[1, 4]
136CLDKEALKDEYDDLSubiquitination[8]
163WESQFTFKYHHVGKLacetylation[10]
163WESQFTFKYHHVGKLubiquitination[5, 8]
172HHVGKLLKEGEEPTVubiquitination[5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [10] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
 Receptor for progesterone (By similarity). 
Sequence Annotation
 DOMAIN 72 171 Cytochrome b5 heme-binding.
 MOD_RES 54 54 Phosphoserine.
 MOD_RES 57 57 Phosphoserine.
 MOD_RES 180 180 Phosphotyrosine (By similarity).
 MOD_RES 181 181 Phosphoserine.  
Keyword
 Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Lipid-binding; Membrane; Microsome; Phosphoprotein; Receptor; Reference proteome; Steroid-binding; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 195 AA 
Protein Sequence
MAAEDVVATG ADPSDLESGG LLHEIFTSPL NLLLLGLCIF LLYKIVRGDQ PAASGDSDDD 60
EPPPLPRLKR RDFTPAELRR FDGVQDPRIL MAINGKVFDV TKGRKFYGPE GPYGVFAGRD 120
ASRGLATFCL DKEALKDEYD DLSDLTAAQQ ETLSDWESQF TFKYHHVGKL LKEGEEPTVY 180
SDEEEPKDES ARKND 195 
Gene Ontology
 GO:0009986; C:cell surface; IDA:UniProtKB.
 GO:0005783; C:endoplasmic reticulum; IDA:HPA.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0005496; F:steroid binding; TAS:ProtInc.
 GO:0007411; P:axon guidance; IEA:Compara. 
Interpro
 IPR001199; Cyt_B5-like_heme/steroid-bd. 
Pfam
 PF00173; Cyt-b5 
SMART
  
PROSITE
 PS00191; CYTOCHROME_B5_1
 PS50255; CYTOCHROME_B5_2 
PRINTS