CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-028416
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Probable ATP-dependent RNA helicase DDX58 
Protein Synonyms/Alias
  
Gene Name
 DDX58 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
89FPQGQKGKVVFFANQubiquitination[1]
104IPVYEQQKSVFSKYFubiquitination[1]
109QQKSVFSKYFERHGYubiquitination[1]
252ELEQVVYKPQKFFRKubiquitination[1]
441RALVDALKNWIEGNPubiquitination[1, 2, 3]
463GILTGRGKTNQNTGMubiquitination[1]
554KEQINMYKEKMMNDSubiquitination[1]
556QINMYKEKMMNDSILubiquitination[1]
585LHIQTHEKFIRDSQEubiquitination[1]
655KQFSSFEKRAKIFCAacetylation[3]
655KQFSSFEKRAKIFCAubiquitination[1]
677WGIHVKYKTFEIPVIubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 722 AA 
Protein Sequence
METSDIQIFY QEDPECQNLS ENSCPPSEVS DTNLYSPFKP RNYQLELALP AMKGKNTIIC 60
APTGCGKTFV SLLICEHHLK KFPQGQKGKV VFFANQIPVY EQQKSVFSKY FERHGYRVTG 120
ISGATAENVP VEQIVENNDI IILTPQILVN NLKKGTIPSL SIFTLMIFDE CHNTSKQHPY 180
NMIMFNYLDQ KLGGSSGPLP QVIGLTASVG VGDAKNTDEA LDYICKLCAS LDASVIATVK 240
HNLEELEQVV YKPQKFFRKV ESRISDKFKY IIAQLMRDTE SLAKRICKDL ENLSQIQNRE 300
FGTQKYEQWI VTVQKACMVF QMPDKDEESR ICKALFLYTS HLRKYNDALI ISEHARMKDA 360
LDYLKDFFSN VRAAGFDEIE QDLTQRFEEK LQELESVSRD PSNENPKLED LCFILQEEYH 420
LNPETITILF VKTRALVDAL KNWIEGNPKL SFLKPGILTG RGKTNQNTGM TLPAQKCILD 480
AFKASGDHNI LIATSVADEG IDIAQCNLVI LYEYVGNVIK MIQTRGRGRA RGSKCFLLTS 540
NAGVIEKEQI NMYKEKMMND SILRLQTWDE AVFREKILHI QTHEKFIRDS QEKPKPVPDK 600
ENKKLLCRKC KALACYTADV RVIEECHYTV LGDAFKECFV SRPHPKPKQF SSFEKRAKIF 660
CARQNCSHDW GIHVKYKTFE IPVIKIESFV VEDIATGVQT LYSKWKDFHF EKIPFDPAEM 720
SK 722 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003690; F:double-stranded DNA binding; IEA:Compara.
 GO:0003725; F:double-stranded RNA binding; IEA:Compara.
 GO:0003727; F:single-stranded RNA binding; IEA:Compara.
 GO:0045087; P:innate immune response; IEA:Compara.
 GO:0032728; P:positive regulation of interferon-beta production; IEA:Compara.
 GO:0043330; P:response to exogenous dsRNA; IEA:Compara.
 GO:0009615; P:response to virus; IEA:Compara. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR021673; RIG-I_C-RD. 
Pfam
 PF00270; DEAD
 PF00271; Helicase_C
 PF11648; RIG-I_C-RD 
SMART
 SM00382; AAA
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS