CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042963
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 E3 ubiquitin-protein ligase HECTD1 
Protein Synonyms/Alias
  
Gene Name
 HECTD1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
120EEEEYETKGGRRRTWubiquitination[1, 2, 3]
134WDDDYVLKRQFSALVubiquitination[3, 4, 5, 6]
285DLITYLQKNADAAFLubiquitination[4]
347SDILNLTKEQPQAKAubiquitination[1, 3]
580VDLGGGLKPPGYYVQubiquitination[5]
634LVDLPISKPFFKLMCubiquitination[1, 3, 4, 5, 6]
651DIKSNMSKLIYESRGubiquitination[5]
730ARFLKEIKDLAIKRRubiquitination[4]
826CMHTGIQKQMEAFRDubiquitination[5]
837AFRDGFNKVFPMEKLubiquitination[1, 5, 6]
950NTCVHYLKLPEYSSEubiquitination[2]
971LLAATMEKGFHLN**ubiquitination[4, 5, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Ligase; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 976 AA 
Protein Sequence
XLSNTLTTSL TSTSSESDTG QEAEYSLYDF LDSCRASTLL AELDDDEDLP EPDEEDDENE 60
DDNQEDQEYE EILRRPSLQR RAGSRSDVTH HAVTSQLPQV PAGAGSRPIG EQEEEEYETK 120
GGRRRTWDDD YVLKRQFSAL VPAFDPRPGR TNVQQTTDLE IPPPGTPHSE LLEEVECTPS 180
PRLALTLKVT GLGTTREVEL PLTNFRSTIF YYVQKLLQLS CNGNVKSDKL RRIWEPTYTI 240
MYREMKDSDK EKENGKMGCW SIEHVEQYLG TDELPKNDLI TYLQKNADAA FLRHWKLTGT 300
NKSIRKNRNC SQLIAAYKDF CEHGTKSGLN QGAISTLQSS DILNLTKEQP QAKAGNGQNS 360
CGVEDVLQLL RILYIVASDP YSRISQEDGD EQLQFTFPPD EFTSKKITTK ILQQIEEPLA 420
LASGALPDWC EQLTSKCPFL IPFETRQLYF TCTAFGASRA IVWLQNRREA TVERTRTTSS 480
VRRDDPGEFR VGRLKHERVK VPRGESLMEW AENVMQIHAD RKSVLEVEFL GEEGTGLGPT 540
LEFYALVAAE FQRTDLGAWL CDDNFPDDES RHVDLGGGLK PPGYYVQRSC GLFTAPFPQD 600
SDELERITKL FHFLGIFLAK CIQDNRLVDL PISKPFFKLM CMGDIKSNMS KLIYESRGDR 660
DLHCTESQSE ASTEEGHDSL SVGSFEEDSK SEFILDPPKP KPPAWFNGIL TWEDFELVNP 720
HRARFLKEIK DLAIKRRQIL SNKGLSEDEK NTKLQELVLK NPSGSGPPLS IEDLGLNFQF 780
CPSSRIYGFT AVDLKPSGED EMITMDNAEE YVDLMFDFCM HTGIQKQMEA FRDGFNKVFP 840
MEKLSSFSHE EVQMILCGNQ SPSWAAEDII NYTEPKLGYT RDSPGFLRFV RVLCGMSSDE 900
RKAFLQFTTG CSTLPPGGLA NLHPRLTVVR KVDATDASYP SVNTCVHYLK LPEYSSEEIM 960
RERLLAATME KGFHLN 976 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:InterPro.
 GO:0016567; P:protein ubiquitination; IEA:GOC. 
Interpro
 IPR000569; HECT. 
Pfam
 PF00632; HECT 
SMART
 SM00119; HECTc 
PROSITE
 PS50237; HECT 
PRINTS