CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015586
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Syntaxin-binding protein 4 
Protein Synonyms/Alias
 Syntaxin 4-interacting protein; STX4-interacting protein; Synip 
Gene Name
 STXBP4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
16VSPSLLEKDPAFQMIubiquitination[1]
27FQMITIAKETGLGLKubiquitination[1]
65CYKDGRLKPGDQLVSubiquitination[2]
156PKTSSTPKTNNDILSubiquitination[2]
168ILSSCEIKTGYNKTVubiquitination[2]
248QQVQADSKGTVSFGDubiquitination[2]
385EAKITELKAQLADYSubiquitination[1]
396ADYSDQNKESVQDLKubiquitination[1]
483ATLALESKELVKSVRubiquitination[2]
487LESKELVKSVRALLDubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane. Inhibits the translocation of SLC2A4 from intracellular vesicles to the plasma membrane by STX4A binding and preventing the interaction between STX4A and VAMP2. Stimulation with insulin disrupts the interaction with STX4A, leading to increased levels of SLC2A4 at the plasma membrane. May also play a role in the regulation of insulin release by pancreatic beta cells after stimulation by glucose (By similarity). 
Sequence Annotation
 DOMAIN 19 105 PDZ.
 DOMAIN 496 529 WW.
 MOD_RES 10 10 Phosphoserine.
 MOD_RES 99 99 Phosphoserine (By similarity).  
Keyword
 3D-structure; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 553 AA 
Protein Sequence
MNKNTSTVVS PSLLEKDPAF QMITIAKETG LGLKVLGGIN RNEGPLVYIQ EIIPGGDCYK 60
DGRLKPGDQL VSVNKESMIG VSFEEAKSII TGAKLRLESA WEIAFIRQKS DNIQPENLSC 120
TSLIEASGEY GPQASTLSLF SSPPEILIPK TSSTPKTNND ILSSCEIKTG YNKTVQIPIT 180
SENSTVGLSN TDVASAWTEN YGLQEKISLN PSVRFKAEKL EMALNYLGIQ PTKEQHQALR 240
QQVQADSKGT VSFGDFVQVA RNLFCLQLDE VNVGAHEISN ILDSQLLPCD SSEADEMERL 300
KCERDDALKE VNTLKEKLLE SDKQRKQLTE ELQNVKQEAK AVVEETRALR SRIHLAEAAQ 360
RQAHGMEMDY EEVIRLLEAK ITELKAQLAD YSDQNKESVQ DLKKRIMVLD CQLRKSEMAR 420
KTFEASTEKL LHFVEAIQEV FSDNSTPLSN LSERRAVLAS QTSLTPLGRN GRSIPATLAL 480
ESKELVKSVR ALLDMDCLPY GWEEAYTADG IKYFINHVTQ TTSWIHPVMS VLNLSRSEEN 540
EEDCSRELPN QKS 553 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0045111; C:intermediate filament cytoskeleton; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0015758; P:glucose transport; IEA:Compara.
 GO:0008286; P:insulin receptor signaling pathway; IEA:Compara.
 GO:0006605; P:protein targeting; IEA:Compara. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR001478; PDZ.
 IPR001202; WW_dom. 
Pfam
 PF00595; PDZ
 PF00397; WW 
SMART
 SM00228; PDZ
 SM00456; WW 
PROSITE
 PS50106; PDZ
 PS01159; WW_DOMAIN_1
 PS50020; WW_DOMAIN_2 
PRINTS