CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002206
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Plasma membrane ATPase 1 
Protein Synonyms/Alias
 Proton pump 1 
Gene Name
 PMA1 
Gene Synonyms/Alias
 YGL008C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
25AHQPTQEKPAKTYDDubiquitination[1]
95LTSDEVLKRRKKYGLacetylation[2]
99EVLKRRKKYGLNQMAacetylation[2]
109LNQMADEKESLVVKFubiquitination[1]
239GESLAVDKHYGDQTFubiquitination[1]
252TFSSSTVKRGEGFMVacetylation[2]
252TFSSSTVKRGEGFMVubiquitination[3]
417LAASRKKKGLDAIDKubiquitination[1]
424KGLDAIDKAFLKSLKacetylation[2]
424KGLDAIDKAFLKSLKubiquitination[1]
428AIDKAFLKSLKQYPKubiquitination[1]
431KAFLKSLKQYPKAKDubiquitination[1]
442KAKDALTKYKVLEFHacetylation[2]
442KAKDALTKYKVLEFHubiquitination[1]
444KDALTKYKVLEFHPFacetylation[2]
444KDALTKYKVLEFHPFubiquitination[1, 3]
456HPFDPVSKKVTAVVEacetylation[2]
456HPFDPVSKKVTAVVEubiquitination[1]
457PFDPVSKKVTAVVESubiquitination[1]
474GERIVCVKGAPLFVLubiquitination[1]
482GAPLFVLKTVEEDHPacetylation[2]
501VHENYENKVAELASRacetylation[2]
501VHENYENKVAELASRubiquitination[1]
555RHLGLRVKMLTGDAVubiquitination[1, 4, 5, 6]
566GDAVGIAKETCRQLGacetylation[2]
566GDAVGIAKETCRQLGubiquitination[1, 4, 5, 6]
615AEVFPQHKYRVVEILubiquitination[1, 3]
643VNDAPSLKKADTGIAacetylation[2]
643VNDAPSLKKADTGIAubiquitination[3]
644NDAPSLKKADTGIAVubiquitination[1, 3, 4, 5, 6]
679SAIIDALKTSRQIFHubiquitination[1]
916RVSTQHEKET*****ubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [4] A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
 Hitchcock AL, Auld K, Gygi SP, Silver PA.
 Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12735-40. [PMID: 14557538]
 [5] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [6] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 
Functional Description
 The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses. 
Sequence Annotation
 ACT_SITE 378 378 4-aspartylphosphate intermediate (By
 METAL 634 634 Magnesium (By similarity).
 METAL 638 638 Magnesium (By similarity).
 MOD_RES 61 61 Phosphoserine.
 MOD_RES 175 175 Phosphothreonine.
 MOD_RES 911 911 Phosphoserine.
 MOD_RES 912 912 Phosphothreonine.
 CROSSLNK 555 555 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 566 566 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 644 644 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Amyloid; ATP-binding; Cell membrane; Complete proteome; Direct protein sequencing; Hydrogen ion transport; Hydrolase; Ion transport; Isopeptide bond; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Prion; Reference proteome; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 918 AA 
Protein Sequence
MTDTSSSSSS SSASSVSAHQ PTQEKPAKTY DDAASESSDD DDIDALIEEL QSNHGVDDED 60
SDNDGPVAAG EARPVPEEYL QTDPSYGLTS DEVLKRRKKY GLNQMADEKE SLVVKFVMFF 120
VGPIQFVMEA AAILAAGLSD WVDFGVICGL LMLNAGVGFV QEFQAGSIVD ELKKTLANTA 180
VVIRDGQLVE IPANEVVPGD ILQLEDGTVI PTDGRIVTED CFLQIDQSAI TGESLAVDKH 240
YGDQTFSSST VKRGEGFMVV TATGDNTFVG RAAALVNKAA GGQGHFTEVL NGIGIILLVL 300
VIATLLLVWT ACFYRTNGIV RILRYTLGIT IIGVPVGLPA VVTTTMAVGA AYLAKKQAIV 360
QKLSAIESLA GVEILCSDKT GTLTKNKLSL HEPYTVEGVS PDDLMLTACL AASRKKKGLD 420
AIDKAFLKSL KQYPKAKDAL TKYKVLEFHP FDPVSKKVTA VVESPEGERI VCVKGAPLFV 480
LKTVEEDHPI PEDVHENYEN KVAELASRGF RALGVARKRG EGHWEILGVM PCMDPPRDDT 540
AQTVSEARHL GLRVKMLTGD AVGIAKETCR QLGLGTNIYN AERLGLGGGG DMPGSELADF 600
VENADGFAEV FPQHKYRVVE ILQNRGYLVA MTGDGVNDAP SLKKADTGIA VEGATDAARS 660
AADIVFLAPG LSAIIDALKT SRQIFHRMYS YVVYRIALSL HLEIFLGLWI AILDNSLDID 720
LIVFIAIFAD VATLAIAYDN APYSPKPVKW NLPRLWGMSI ILGIVLAIGS WITLTTMFLP 780
KGGIIQNFGA MNGIMFLQIS LTENWLIFIT RAAGPFWSSI PSWQLAGAVF AVDIIATMFT 840
LFGWWSENWT DIVTVVRVWI WSIGIFCVLG GFYYEMSTSE AFDRLMNGKP MKEKKSTRSV 900
EDFMAAMQRV STQHEKET 918 
Gene Ontology
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0045121; C:membrane raft; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008553; F:hydrogen-exporting ATPase activity, phosphorylative mechanism; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006754; P:ATP biosynthetic process; IEA:InterPro.
 GO:0006885; P:regulation of pH; IEP:SGD. 
Interpro
 IPR004014; ATPase_P-typ_cation-transptr_N.
 IPR023299; ATPase_P-typ_cyto_domN.
 IPR018303; ATPase_P-typ_P_site.
 IPR023298; ATPase_P-typ_TM_dom.
 IPR008250; ATPase_P-typ_transduc_dom_A.
 IPR001757; Cation_transp_P_typ_ATPase.
 IPR006534; H+_ATPase_P-typ_IIIA.
 IPR023214; HAD-like_dom. 
Pfam
 PF00690; Cation_ATPase_N
 PF00122; E1-E2_ATPase
 PF00702; Hydrolase 
SMART
 SM00831; Cation_ATPase_N 
PROSITE
 PS00154; ATPASE_E1_E2 
PRINTS
 PR00119; CATATPASE.
 PR00120; HATPASE.