CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017183
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Lanosterol 14-alpha demethylase 
Protein Synonyms/Alias
 LDM; CYPLI; Cytochrome P450 51A1; Cytochrome P450-14DM; Cytochrome P45014DM; Cytochrome P450LI; Sterol 14-alpha demethylase 
Gene Name
 Cyp51a1 
Gene Synonyms/Alias
 Cyp51 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
141LTTPVFGKGVAYDVPubiquitination[1]
156NAIFLEQKKIIKSGLacetylation[2]
156NAIFLEQKKIIKSGLubiquitination[1]
157AIFLEQKKIIKSGLNubiquitination[1]
160LEQKKIIKSGLNIAHubiquitination[1]
169GLNIAHFKQYVPIIEubiquitination[1]
177QYVPIIEKEAKEYFQubiquitination[1]
180PIIEKEAKEYFQSWGubiquitination[1]
261DRAHREIKNIFYKAIubiquitination[1]
266EIKNIFYKAIQKRRLubiquitination[1]
275IQKRRLSKEPAEDILubiquitination[1]
336KDKPLQEKCYLEQKAubiquitination[1]
342EKCYLEQKAVCGEDLubiquitination[1]
358PLTYDQLKDLNLLDRubiquitination[1]
385MTMMRMAKTPQTVAGubiquitination[1]
412PTVNQRLKDSWAERLubiquitination[1]
436DNPASGEKFAYVPFGacetylation[2]
436DNPASGEKFAYVPFGubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
 Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol (By similarity). 
Sequence Annotation
 METAL 449 449 Iron (heme axial ligand) (By similarity).  
Keyword
 Cholesterol biosynthesis; Cholesterol metabolism; Complete proteome; Endoplasmic reticulum; Heme; Iron; Lipid biosynthesis; Lipid metabolism; Membrane; Metal-binding; Microsome; Monooxygenase; NADP; Oxidoreductase; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 503 AA 
Protein Sequence
MVLLGLLQSG GWVLGQAMEQ VTGGNLLSTL LIACAFTLSL VYLFRLAVGH MVQLPAGAKS 60
PPHIYSPIPF LGHAIAFGKS PIEFLENAYE KYGPVFSFTM VGKTFTYLLG SDAAALLFNS 120
KNEDLNAEEV YGRLTTPVFG KGVAYDVPNA IFLEQKKIIK SGLNIAHFKQ YVPIIEKEAK 180
EYFQSWGESG ERNVFEALSE LIILTASHCL HGKEIRSQLN EKVAQLYADL DGGFTHAAWL 240
LPAWLPLPSF RRRDRAHREI KNIFYKAIQK RRLSKEPAED ILQTLLDSTY KDGRPLTDEE 300
ISGMLIGLLL AGQHTSSTTS AWMGFFLAKD KPLQEKCYLE QKAVCGEDLP PLTYDQLKDL 360
NLLDRCIKET LRLRPPIMTM MRMAKTPQTV AGYTIPPGHQ VCVSPTVNQR LKDSWAERLD 420
FNPDRYLQDN PASGEKFAYV PFGAGRHRCV GENFAYVQIK TIWSTMLRLY EFDLINGYFP 480
TVNYTTMIHT PENPVIRYKR RSK 503 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0020037; F:heme binding; ISS:UniProtKB.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0008398; F:sterol 14-demethylase activity; ISS:UniProtKB.
 GO:0006695; P:cholesterol biosynthetic process; TAS:MGI.
 GO:0033488; P:cholesterol biosynthetic process via 24,25-dihydrolanosterol; IEA:Compara. 
Interpro
 IPR001128; Cyt_P450.
 IPR017972; Cyt_P450_CS.
 IPR002403; Cyt_P450_E_grp-IV. 
Pfam
 PF00067; p450 
SMART
  
PROSITE
 PS00086; CYTOCHROME_P450 
PRINTS
 PR00465; EP450IV.
 PR00385; P450.