UniProt Accession

 RABX5_HUMAN; Q9UJ41; Q3HKR2; Q3HKR3; Q53FG0 

Genbank Protein ID

 AJ250042; DQ230533; DQ230534; AK127790; BT007107; AK223329; AC027644; BC015330 

Genbank Nucleotide ID

 CAB57359.1; ABA64473.1; ABA64474.1; BAC87138.1; AAP35771.1; BAD97049.1; AAQ93362.1; AAH15330.1 

Protein Name

 Rab5 GDP/GTP exchange factor 

Protein Synonyms/Alias

 RAP1; Rabaptin-5-associated exchange factor for Rab5; Rabex-5 

Gene Name

 RABGEF1 

Gene Synonyms/Alias

 RABEX5 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
85	AQSLTFSKFEEKKTN	ubiquitination	[1, 2]
89	TFSKFEEKKTNEKTR	ubiquitination	[2]
103	RKVTTVKKFFSASSR	ubiquitination	[2]
121	KKEIQEAKAPSPSIN	ubiquitination	[2]
151	EFLKTFHKTGQEIYK	ubiquitination	[2]
158	KTGQEIYKQTKLFLE	ubiquitination	[2]
213	KIMDQIEKYIMTRLY	acetylation	[3]

Reference

 [1] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 

Functional Description

 Rab effector protein acting as linker between gamma- adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase (By similarity). 

Sequence Annotation

 DOMAIN 449 592 VPS9.
 ZN_FING 151 185 A20-type.
 REGION 139 252 Interaction with ubiquitinated proteins.  

Keyword

 3D-structure; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Endocytosis; Endosome; Metal-binding; Protein transport; Reference proteome; Transport; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 708 AA 

Protein Sequence

Gene Ontology

 GO:0005769; C:early endosome; IEA:UniProtKB-SubCell.
 GO:0055037; C:recycling endosome; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0017112; F:Rab guanyl-nucleotide exchange factor activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 

Interpro

 IPR003123; VPS9.
 IPR013995; VPS9_subgr.
 IPR002653; Znf_A20. 

Pfam

 PF02204; VPS9
 PF01754; zf-A20 

SMART

 SM00167; VPS9
 SM00259; ZnF_A20 

PROSITE

 PS51205; VPS9
 PS51036; ZF_A20 

PRINTS