CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002102
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phenylalanine-4-hydroxylase 
Protein Synonyms/Alias
 PAH; Phe-4-monooxygenase 
Gene Name
 Pah 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
50EEVGALAKVLRLFEEacetylation[1]
96PVLGSIIKSLRNDIGacetylation[1]
195GTVFRTLKALYKTHAacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
  
Sequence Annotation
 DOMAIN 36 114 ACT.
 METAL 285 285 Iron.
 METAL 290 290 Iron.
 METAL 330 330 Iron.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 16 16 Phosphoserine; by PKA.  
Keyword
 3D-structure; Acetylation; Allosteric enzyme; Complete proteome; Direct protein sequencing; Iron; Metal-binding; Monooxygenase; Oxidoreductase; Phenylalanine catabolism; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 453 AA 
Protein Sequence
MAAVVLENGV LSRKLSDFGQ ETSYIEDNSN QNGAISLIFS LKEEVGALAK VLRLFEENDI 60
NLTHIESRPS RLNKDEYEFF TYLDKRTKPV LGSIIKSLRN DIGATVHELS RDKEKNTVPW 120
FPRTIQELDR FANQILSYGA ELDADHPGFK DPVYRARRKQ FADIAYNYRH GQPIPRVEYT 180
EEEKQTWGTV FRTLKALYKT HACYEHNHIF PLLEKYCGFR EDNIPQLEDV SQFLQTCTGF 240
RLRPVAGLLS SRDFLGGLAF RVFHCTQYIR HGSKPMYTPE PDICHELLGH VPLFSDRSFA 300
QFSQEIGLAS LGAPDEYIEK LATIYWFTVE FGLCKEGDSI KAYGAGLLSS FGELQYCLSD 360
KPKLLPLELE KTACQEYSVT EFQPLYYVAE SFSDAKEKVR TFAATIPRPF SVRYDPYTQR 420
VEVLDNTQQL KILADSINSE VGILCNALQK IKS 453 
Gene Ontology
 GO:0016597; F:amino acid binding; IDA:RGD.
 GO:0048037; F:cofactor binding; IDA:RGD.
 GO:0005506; F:iron ion binding; IDA:RGD.
 GO:0004505; F:phenylalanine 4-monooxygenase activity; IDA:RGD.
 GO:0042803; F:protein homodimerization activity; IDA:RGD.
 GO:0006559; P:L-phenylalanine catabolic process; IEA:UniProtKB-UniPathway.
 GO:0006558; P:L-phenylalanine metabolic process; IDA:RGD.
 GO:0018126; P:protein hydroxylation; IDA:RGD.
 GO:0046146; P:tetrahydrobiopterin metabolic process; IDA:RGD.
 GO:0006571; P:tyrosine biosynthetic process; IDA:RGD. 
Interpro
 IPR002912; ACT_dom.
 IPR001273; ArAA_hydroxylase.
 IPR018301; ArAA_hydroxylase_Fe/CU_BS.
 IPR019774; Aromatic-AA_hydroxylase_C.
 IPR005961; Phe-4-hydroxylase_tetra.
 IPR019773; Tyrosine_3-monooxygenase-like. 
Pfam
 PF01842; ACT
 PF00351; Biopterin_H 
SMART
  
PROSITE
 PS51671; ACT
 PS00367; BH4_AAA_HYDROXYL_1
 PS51410; BH4_AAA_HYDROXYL_2 
PRINTS
 PR00372; FYWHYDRXLASE.