CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008833
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Magnesium-activated aldehyde dehydrogenase, cytosolic 
Protein Synonyms/Alias
 Mg(2+)-activated acetaldehyde dehydrogenase; Mg(2+)-ACDH 
Gene Name
 ALD6 
Gene Synonyms/Alias
 ALDH1; YPL061W; LPE9 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
3*****MTKLHFDTAEubiquitination[1, 2, 3, 4]
33TGLFINNKFMKAQDGubiquitination[5]
95ERGRLLSKLADELESubiquitination[5]
142DAAAYADKVNGRTINacetylation[6]
142DAAAYADKVNGRTINubiquitination[4, 5]
196MGNVCILKPAAVTPLubiquitination[4]
213LYFASLCKKVGIPAGacetylation[6]
214YFASLCKKVGIPAGVubiquitination[5]
244TNDPRIRKLAFTGSTacetylation[6]
255TGSTEVGKSVAVDSSubiquitination[5]
267DSSESNLKKITLELGacetylation[6]
276ITLELGGKSAHLVFDacetylation[6]
288VFDDANIKKTLPNLVacetylation[6]
300NLVNGIFKNAGQICSacetylation[6]
342KVGNPFDKANFQGAIacetylation[6]
342KVGNPFDKANFQGAIubiquitination[5]
366MNYIDIGKKEGAKILacetylation[6]
378KILTGGEKVGDKGYFacetylation[6]
378KILTGGEKVGDKGYFubiquitination[5]
402NEDMRIVKEEIFGPVacetylation[6, 7]
414GPVVTVAKFKTLEEGacetylation[6]
452LKVAKMLKAGTVWINubiquitination[5]
474RVPFGGVKQSGYGREacetylation[6]
474RVPFGGVKQSGYGREubiquitination[5]
494YHAYTEVKAVRIKL*acetylation[6]
Reference
 [1] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [2] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [4] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [5] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [6] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [7] Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures.
 Xie H, Bandhakavi S, Roe MR, Griffin TJ.
 J Proteome Res. 2007 May;6(5):2019-26. [PMID: 17397211] 
Functional Description
  
Sequence Annotation
 NP_BIND 249 254 NAD (By similarity).
 ACT_SITE 272 272 Proton acceptor (By similarity).
 ACT_SITE 306 306 Nucleophile (By similarity).
 CROSSLNK 3 3 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Complete proteome; Cytoplasm; Direct protein sequencing; Isopeptide bond; Magnesium; NAD; Oxidoreductase; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 500 AA 
Protein Sequence
MTKLHFDTAE PVKITLPNGL TYEQPTGLFI NNKFMKAQDG KTYPVEDPST ENTVCEVSSA 60
TTEDVEYAIE CADRAFHDTE WATQDPRERG RLLSKLADEL ESQIDLVSSI EALDNGKTLA 120
LARGDVTIAI NCLRDAAAYA DKVNGRTINT GDGYMNFTTL EPIGVCGQII PWNFPIMMLA 180
WKIAPALAMG NVCILKPAAV TPLNALYFAS LCKKVGIPAG VVNIVPGPGR TVGAALTNDP 240
RIRKLAFTGS TEVGKSVAVD SSESNLKKIT LELGGKSAHL VFDDANIKKT LPNLVNGIFK 300
NAGQICSSGS RIYVQEGIYD ELLAAFKAYL ETEIKVGNPF DKANFQGAIT NRQQFDTIMN 360
YIDIGKKEGA KILTGGEKVG DKGYFIRPTV FYDVNEDMRI VKEEIFGPVV TVAKFKTLEE 420
GVEMANSSEF GLGSGIETES LSTGLKVAKM LKAGTVWINT YNDFDSRVPF GGVKQSGYGR 480
EMGEEVYHAY TEVKAVRIKL 500 
Gene Ontology
 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0004029; F:aldehyde dehydrogenase (NAD) activity; IEA:EC.
 GO:0004030; F:aldehyde dehydrogenase [NAD(P)+] activity; IDA:SGD.
 GO:0019413; P:acetate biosynthetic process; IMP:SGD.
 GO:0006068; P:ethanol catabolic process; IEA:UniProtKB-UniPathway.
 GO:0006740; P:NADPH regeneration; IGI:SGD.
 GO:0009651; P:response to salt stress; IMP:SGD. 
Interpro
 IPR016161; Ald_DH/histidinol_DH.
 IPR016163; Ald_DH_C.
 IPR016160; Ald_DH_CS.
 IPR016162; Ald_DH_N.
 IPR015590; Aldehyde_DH_dom. 
Pfam
 PF00171; Aldedh 
SMART
  
PROSITE
 PS00070; ALDEHYDE_DEHYDR_CYS
 PS00687; ALDEHYDE_DEHYDR_GLU 
PRINTS