UniProt Accession

 TFS2_YEAST; P07273; D6VU96; P24535 

Genbank Protein ID

 M36724; D12478; M60770; Z72565; X00047; BK006941 

Genbank Nucleotide ID

 AAA88734.1; BAA02046.1; AAA34580.1; CAA96744.1; CAA24928.1; DAA08057.1 

Protein Name

 Transcription elongation factor S-II 

Protein Synonyms/Alias

 DNA strand transfer protein alpha; STP-alpha; DNA strand transferase 1; Pyrimidine pathway regulatory protein 2 

Gene Name

 DST1 

Gene Synonyms/Alias

 PPR2; YGL043W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
39	KEFVPTEKLLRETKV	acetylation	[1]
184	AIESEMNKVNNCDTN	ubiquitination	[2]
278	CGKCKEKKVSYYQLQ	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. 

Sequence Annotation

 DOMAIN 5 79 TFIIS N-terminal.
 DOMAIN 148 264 TFIIS central.
 ZN_FING 267 307 TFIIS-type.
 MOD_RES 116 116 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 309 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IDA:SGD.
 GO:0001139; F:core RNA polymerase II recruiting transcription factor activity; IMP:SGD.
 GO:0001012; F:RNA polymerase II regulatory region DNA binding; IDA:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0001193; P:maintenance of transcriptional fidelity during DNA-dependent transcription elongation from RNA polymerase II promoter; IMP:SGD.
 GO:0006379; P:mRNA cleavage; IDA:SGD.
 GO:0045899; P:positive regulation of RNA polymerase II transcriptional preinitiation complex assembly; IDA:SGD.
 GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IDA:SGD.
 GO:0031440; P:regulation of mRNA 3'-end processing; IMP:SGD.
 GO:0031564; P:transcription antitermination; IDA:SGD.
 GO:0006362; P:transcription elongation from RNA polymerase I promoter; IDA:SGD.
 GO:0042797; P:tRNA transcription from RNA polymerase III promoter; IMP:SGD. 

Interpro

 IPR016492; TF_IIS-rel.
 IPR003617; TFIIS/CRSP70_N_sub.
 IPR003618; TFIIS_cen_dom.
 IPR017923; TFIIS_N.
 IPR017890; TFS2M.
 IPR006289; TFSII.
 IPR001222; Znf_TFIIS. 

Pfam

 PF08711; Med26
 PF01096; TFIIS_C
 PF07500; TFIIS_M 

SMART

 SM00510; TFS2M
 SM00509; TFS2N
 SM00440; ZnF_C2C2 

PROSITE

 PS51321; TFIIS_CENTRAL
 PS51319; TFIIS_N
 PS00466; ZF_TFIIS_1
 PS51133; ZF_TFIIS_2 

PRINTS