UniProt Accession

 NELFA_HUMAN; Q9H3P2; A2A2T1; O95392 

Genbank Protein ID

 AF101434; AB044549; AF131751; AK126056; AL132868; CH471131; BC002764 

Genbank Nucleotide ID

 AAC72982.1; BAB18651.1; AAD20034.1; BAG54283.1; CAM15219.1; EAW82546.1; AAH02764.2 

Protein Name

 Negative elongation factor A 

Protein Synonyms/Alias

 NELF-A; Wolf-Hirschhorn syndrome candidate 2 protein 

Gene Name

 NELFA 

Gene Synonyms/Alias

 WHSC2; P/OKcl.15 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
127	ILGELREKVGECEAS	ubiquitination	[1]
161	GPLTPPVKHFQLKRK	ubiquitination	[1]
190	TETAQQLKRSAGVPF	ubiquitination	[1]
371	PTLPAQFKQRAPMYN	ubiquitination	[2, 3]
446	FAAQEMFKTANKVTR	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex. 

Sequence Annotation

 DOMAIN 189 248 HDAg-like.
 MOD_RES 157 157 Phosphothreonine.
 MOD_RES 277 277 Phosphothreonine.
 MOD_RES 363 363 Phosphoserine.  

Keyword

 Alternative splicing; Complete proteome; Direct protein sequencing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 528 AA 

Protein Sequence

Gene Ontology

 GO:0032021; C:NELF complex; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0007275; P:multicellular organismal development; TAS:ProtInc.
 GO:0034244; P:negative regulation of transcription elongation from RNA polymerase II promoter; IEA:Compara.
 GO:0050434; P:positive regulation of viral transcription; TAS:Reactome.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 

Interpro

 IPR026305; NELF-A. 

Pfam

  

SMART

  

PROSITE

  

PRINTS