CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021307
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sodium-dependent neutral amino acid transporter B(0)AT2 
Protein Synonyms/Alias
 Sodium- and chloride-dependent neurotransmitter transporter NTT73; Sodium-coupled branched-chain amino-acid transporter 1; Solute carrier family 6 member 15; Transporter v7-3 
Gene Name
 SLC6A15 
Gene Synonyms/Alias
 B0AT2; NTT73; SBAT1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
21DDVTESVKDLLSNEDubiquitination[1, 2, 3, 4]
35DAADDAFKTSELIVDubiquitination[1, 3, 4]
658NLASVTYKRGRVLKEubiquitination[1, 4, 5, 6]
664YKRGRVLKEPVNLEGubiquitination[1, 3]
680DTSLIHGKIPSEMPSubiquitination[4]
692MPSPNFGKNIYRKQSubiquitination[1, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Functions as a sodium-dependent neutral amino acid transporter. Exhibits preference for the branched-chain amino acids, particularly leucine, valine and isoleucine and methionine. Mediates the saturable, pH-sensitive and electrogenic cotransport of proline and sodium ions with a stoichiometry of 1:1. May have a role as transporter for neurotransmitter precursors into neurons. In contrast to other members of the neurotransmitter transporter family, does not appear to be chloride-dependent. 
Sequence Annotation
 MOD_RES 55 55 Phosphoserine.
 MOD_RES 687 687 Phosphoserine.
 CARBOHYD 187 187 N-linked (GlcNAc...) (Potential).
 CARBOHYD 213 213 N-linked (GlcNAc...) (Potential).
 CARBOHYD 383 383 N-linked (GlcNAc...) (Potential).
 CARBOHYD 394 394 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Amino-acid transport; Complete proteome; Glycoprotein; Ion transport; Membrane; Neurotransmitter transport; Phosphoprotein; Polymorphism; Reference proteome; Sodium; Sodium transport; Symport; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 730 AA 
Protein Sequence
MPKNSKVVKR ELDDDVTESV KDLLSNEDAA DDAFKTSELI VDGQEEKDTD VEEGSEVEDE 60
RPAWNSKLQY ILAQVGFSVG LGNVWRFPYL CQKNGGGAYL LPYLILLMVI GIPLFFLELS 120
VGQRIRRGSI GVWNYISPKL GGIGFASCVV CYFVALYYNV IIGWSLFYFS QSFQQPLPWD 180
QCPLVKNASH TFVEPECEQS SATTYYWYRE ALNISSSISE SGGLNWKMTI CLLAAWVMVC 240
LAMIKGIQSS GKIIYFSSLF PYVVLICFLI RAFLLNGSID GIRHMFTPKL EIMLEPKVWR 300
EAATQVFFAL GLGFGGVIAF SSYNKRDNNC HFDAVLVSFI NFFTSVLATL VVFAVLGFKA 360
NVINEKCITQ NSETIMKFLK MGNISQDIIP HHINLSTVTA EDYHLVYDII QKVKEEEFPA 420
LHLNSCKIEE ELNKAVQGTG LAFIAFTEAM THFPASPFWS VMFFLMLVNL GLGSMFGTIE 480
GIVTPIVDTF KVRKEILTVI CCLLAFCIGL IFVQRSGNYF VTMFDDYSAT LPLLIVVILE 540
NIAVCFVYGI DKFMEDLKDM LGFAPSRYYY YMWKYISPLM LLSLLIASVV NMGLSPPGYN 600
AWIEDKASEE FLSYPTWGLV VCVSLVVFAI LPVPVVFIVR RFNLIDDSSG NLASVTYKRG 660
RVLKEPVNLE GDDTSLIHGK IPSEMPSPNF GKNIYRKQSG SPTLDTAPNG RYGIGYLMAD 720
IMPDMPESDL 730 
Gene Ontology
 GO:0016021; C:integral to membrane; NAS:UniProtKB.
 GO:0005887; C:integral to plasma membrane; IEA:InterPro.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0005326; F:neurotransmitter transporter activity; NAS:UniProtKB.
 GO:0005328; F:neurotransmitter:sodium symporter activity; IEA:InterPro.
 GO:0005298; F:proline:sodium symporter activity; IEA:Compara.
 GO:0015820; P:leucine transport; ISS:UniProtKB.
 GO:0015824; P:proline transport; ISS:UniProtKB.
 GO:0055085; P:transmembrane transport; TAS:Reactome. 
Interpro
 IPR000175; Na/ntran_symport.
 IPR002438; Na/ntran_symport_orphan. 
Pfam
 PF00209; SNF 
SMART
  
PROSITE
 PS00610; NA_NEUROTRAN_SYMP_1
 PS00754; NA_NEUROTRAN_SYMP_2
 PS50267; NA_NEUROTRAN_SYMP_3 
PRINTS
 PR00176; NANEUSMPORT.
 PR01206; ORPHTRNSPORT.