UniProt Accession

 PYRE_ECOLI; P0A7E3; P00495; Q2M7V6 

Genbank Protein ID

 X00781; V01578; L10328; U00096; AP009048 

Genbank Nucleotide ID

 CAA25358.1; CAA24899.1; AAA61995.1; AAC76666.1; BAE77650.1 

Protein Name

 Orotate phosphoribosyltransferase 

Protein Synonyms/Alias

 OPRT; OPRTase 

Gene Name

 pyrE 

Gene Synonyms/Alias

 b3642; JW3617 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
19	ALSKQVLKFGEFTLK	acetylation	[1]
26	KFGEFTLKSGRKSPY	acetylation	[1]
103	CFNRKEAKDHGEGGN	acetylation	[1]
194	LIAYLEEKPEMAEHL	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) (By similarity). 

Sequence Annotation

 REGION 34 35 Orotate binding (By similarity).
 REGION 72 73 5-phosphoribose 1-diphosphate binding.
 REGION 124 132 5-phosphoribose 1-diphosphate binding (By
 BINDING 26 26 5-phosphoribose 1-diphosphate (By
 BINDING 99 99 5-phosphoribose 1-diphosphate; shared
 BINDING 100 100 5-phosphoribose 1-diphosphate.
 BINDING 103 103 5-phosphoribose 1-diphosphate; shared
 BINDING 105 105 5-phosphoribose 1-diphosphate; shared
 BINDING 128 128 Orotate (By similarity).
 BINDING 156 156 Orotate (By similarity).  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; Glycosyltransferase; Magnesium; Pyrimidine biosynthesis; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 213 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:EcoliWiki.
 GO:0000287; F:magnesium ion binding; IDA:EcoCyc.
 GO:0004588; F:orotate phosphoribosyltransferase activity; IDA:EcoCyc.
 GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IMP:EcoCyc.
 GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0006221; P:pyrimidine nucleotide biosynthetic process; IMP:EcoliWiki. 

Interpro

 IPR023031; OPRT.
 IPR004467; Or_phspho_trans_dom.
 IPR000836; PRibTrfase_dom. 

Pfam

 PF00156; Pribosyltran 

SMART

  

PROSITE

 PS00103; PUR_PYR_PR_TRANSFER 

PRINTS