| Tag | Content |
|---|
| CPLM ID | CPLM-017895 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Ubiquitin carboxyl-terminal hydrolase 33 |
Protein Synonyms/Alias | Deubiquitinating enzyme 33; Ubiquitin thioesterase 33; Ubiquitin-specific-processing protease 33; VHL-interacting deubiquitinating enzyme 1 |
Gene Name | Usp33 |
Gene Synonyms/Alias | Vdu1 |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 34 | LGACQDCKVRGPNLW | ubiquitination | [1] | | 227 | ANLFQGIKTVNPTFR | ubiquitination | [1] | | 342 | KVCNKINKANADVEL | ubiquitination | [1] | | 849 | NTKIAVTKCGSVMLK | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta- arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. |
Sequence Annotation | DOMAIN 684 777 DUSP 1.
DOMAIN 785 888 DUSP 2.
ZN_FING 28 92 UBP-type.
ACT_SITE 163 163 Nucleophile (By similarity).
ACT_SITE 640 640 Proton acceptor (By similarity).
MOD_RES 407 407 Phosphoserine (By similarity). |
Keyword | Alternative splicing; Complete proteome; Cytoplasm; Cytoskeleton; Endocytosis; Hydrolase; Metal-binding; Phosphoprotein; Protease; Reference proteome; Repeat; Thiol protease; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 909 AA |
Protein Sequence | MTTFRNHCPH LDSVGEITKE DLIQKSLGAC QDCKVRGPNL WACLENRCSY VGCGESQVDH 60
STIHSQETKH YLTVNLTTLR VWCYACSKEV FLDRKLGTPP SLPHVRQPQQ TQENSVQDFK 120
IPSNPALKTP MVAVSEDLDI EVEEEDELKA RGLTGLKNIG NTCYMNAALQ ALSNCPPLTQ 180
FFLDCGGLAR TDKKPAICKS YLKLMTELWH KSRPGSVVPA NLFQGIKTVN PTFRGYSQQD 240
AQEFLRCLMD LLHEELKEQV MEMEEEPQTL TSEETVEEEK SQSDVDFQSC ESCSSSEKAE 300
NESGSKGFPE DSNETTMLIQ DEDDLEMAKD WQKEKVCNKI NKANADVELD KDRDTVCETV 360
DLNSQETVKV QIHGRASESI TDVHLNDLAT SQILPSNESV SPRLSASPPK LGSLWPGLSP 420
PHKKAQSTSA KRKKQHKKYR SVISDIFDGT VISSVQCLTC DRVSITLETF QDLSLPIPGK 480
EDLAKLHSSS HPTIVKAGSC GEAYAPQGWI AFFMEYVKRF VVSCVPSWFW GPVVTLQDCL 540
AAFFARDELK GDNMYSCEKC KKLRNGVKFC KVQKFPEILC IHLKRFRHEL MFSTKISTHV 600
SFPLEGLDLQ PFLAKDSPAQ IVTYDLLSVI CHHGTASSGH YIAYCRNNLN NLWYEFDDQS 660
VTEVSESTVQ NAEAYVLFYR KSSEEAQKER RRISNLLNIM EPSLLQFYIS RQWLNKFKTF 720
AEPGPISNND FLCIHGGIPP RKASYIEDLV LMLPQNIWDN LYSRYGGGPA VNHLYICHTC 780
QIELEKIEKR RKTELEIFIR LNRAFQEEDS PATFYCISMQ WFREWESFVK GKDGDPPGPI 840
DNTKIAVTKC GSVMLKQGAD SGQISEETWN FLQSIYGGGP EVILRPPVVH VDPDVLQAEE 900
KIEVETRSL 909 |
Gene Ontology | GO:0044297; C:cell body; IDA:MGI. GO:0005813; C:centrosome; ISS:UniProtKB. GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell. GO:0004197; F:cysteine-type endopeptidase activity; ISS:UniProtKB. GO:0004221; F:ubiquitin thiolesterase activity; ISS:UniProtKB. GO:0004843; F:ubiquitin-specific protease activity; ISS:UniProtKB. GO:0008270; F:zinc ion binding; ISS:UniProtKB. GO:0007411; P:axon guidance; IMP:UniProtKB. GO:0016477; P:cell migration; IMP:UniProtKB. GO:0051298; P:centrosome duplication; ISS:UniProtKB. GO:0006897; P:endocytosis; IEA:UniProtKB-KW. GO:0071108; P:protein K48-linked deubiquitination; ISS:UniProtKB. GO:0070536; P:protein K63-linked deubiquitination; ISS:UniProtKB. GO:0008277; P:regulation of G-protein coupled receptor protein signaling pathway; ISS:UniProtKB. GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. |
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