CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-043115
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Eukaryotic translation initiation factor 2-alpha kinase 4 
Protein Synonyms/Alias
  
Gene Name
 EIF2AK4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
416AVIKVQNKLDGCCYAubiquitination[1]
425DGCCYAVKRIPINPAubiquitination[1]
440SRQFRRIKGEVTLLSubiquitination[1, 2]
913RNNILNLKRYCIERVubiquitination[1]
1037NSLCRLYKFIEQKGDacetylation[2, 3, 4]
1267LVDHVLQKLRTKVTDubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1427 AA 
Protein Sequence
XAKQERLEIA SLSNQDHTSK KDPGGHRTAA ILHGGSPDFV GNGKHRANSS GRSRRERQYS 60
VCNSEDSPGS CEILYFNMGS PDQLMVHKGK CIGSDEQLGK LVYNALETAT GGFVLLYEWV 120
LQWQKKMGPF LTSQEKEKID KCKKQIQGTE TEFNSLVKLS HPNVVRYLAM NLKEQDDSIV 180
VDILVEHISG VSLAAHLSHS GPIPVHQLRR YTAQLLSGLD YLHSNSVVHK VLSASNVLVD 240
AEGTVKITDY SISKRLADIC KEDVFEQTRV RFSDNALPYK TGKKGDVWRL GLLLLSLSQG 300
QECGEYPVTI PSDLPADFQD FLKKCVCLDD KERWSPQQLL KHSFINPQPK MPLVEQSPED 360
SEGQDYVETV IPSNRLPSAA FFSETQRQFS RYFIEFEELQ LLGKGAFGAV IKVQNKLDGC 420
CYAVKRIPIN PASRQFRRIK GEVTLLSRLH HENIVRYYNA WIERHERPAG PGTPPPDSGP 480
LAKDDRAARG QPASDTDGLD SVEAAAPPPI LSSSVEWSTS GERSASARFP ATGPGSSDDE 540
DDDEDEHGGV FSQSFLPASD SESDIIFDNE DENSKSQNQM EYCEKSTLRD TIDQGLYRDT 600
VRLWRLFREI LDGLAYIHEK GMIHRDLKPV NIFLDSDDHV KIGDFGLATD HLAFSADSKQ 660
DDQTGDLIKS DPSGHLTGMV GTALYVSPEV QGSTKSAYNQ KVDLFSLGII FFEMSYHPMV 720
TASERIFVLN QLRDPTSPKF PEDFDDGEHA KQKSVISWLL NHDPAKRPTA TELLKSELLP 780
PPQMEESELH EVLHHTLTNV DGKAYRTMMA QIFSQRISPA IDYTYDSDIL KGNFSIRTAK 840
MQQHVCETII RIFKRHGAVQ LCTPLLLPRN RQIYEHNEAA LFMDHSGMLV MLPFDLRIPF 900
ARYVARNNIL NLKRYCIERV FRPRKLDRFH PKELLECAFD IVTSTTNSFL PTAEIIYTIY 960
EIIQEFPALQ ERNYSIYLNH TMLLKAILLH CGIPEDKLSQ VYIILYDAVT EKLTRREVEA 1020
KFCNLSLSSN SLCRLYKFIE QKGDLQDLMP TINSLIKQKT GIAQLVKYGL KDLEEVVGLL 1080
KKLGIKLQVL INLGLVYKVQ QHNGIIFQFV AFIKRRQRAV PEILAAGGRY DLLIPQFRGP 1140
QALGPVPTAI GVSIAIDKIS AAVLNMEESV TISSCDLLVV SVGQMSMSRA INLTQKLWTA 1200
GITAEIMYDW SQSQEELQEY CRHHEITYVA LVSDKEGSHV KVKSFEKERQ TEKRVLETEL 1260
VDHVLQKLRT KVTDERNGRE ASDNLAVQNL KGSFSNASGL FEIHGATVVP IVSVLAPEKL 1320
SASTRRRYET QVQTRLQTSL ANLHQKSSEI EILAVDLPKE TILQFLSLEW DADEQAFNTT 1380
VKQLLSRLPK QRYLKLVCDE IYNIKVEKKV SVLFLYSYRD DYYRILF 1427 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004694; F:eukaryotic translation initiation factor 2alpha kinase activity; IEA:InterPro. 
Interpro
 IPR004154; Anticodon-bd.
 IPR026920; EIF2AK4.
 IPR024435; HisRS-related_dom.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF12745; HGTP_anticodon2
 PF00069; Pkinase 
SMART
  
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS